High efficiency separation of microbial aggregates using capillary electrophoresis

被引：61

作者：

Schneiderheinze, JM ^{[1
]}

Armstrong, DW ^{[1
]}

Schulte, G ^{[1
]}

Westenberg, DJ ^{[1
]}

机构：

[1] Univ Missouri, Dept Chem, Rolla, MO 65401 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 189卷 / 01期

关键词：

capillary electrophoresis characterization; polymer; bacterium; yeast; separation;

D O I：

10.1111/j.1574-6968.2000.tb09203.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis limes under 10 min and efficiencies approaching 1 000 000 plates m(-1) in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible with brief immersion in an ultrasound bath. This reversibility was confirmed by Visual microscopy and electrophoretic data. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：39 / 44

页数：6

共 20 条

[1] HIGH-VOLTAGE CAPILLARY ZONE ELECTROPHORESIS OF RED BLOOD-CELLS [J].