Annexins II and V inhibit cell migration

Cell motility is a crucial component involved in wound healing, development, and tumor metastasis. This study investigated whether extracellular annexins, members of a calcium- and phospholipid-binding family of proteins, play a role in the migration of Lewis lung carcinoma cells. Using assays for wound closure and migration through 8-mu m pores, it was found that annexins II and V significantly (>40%) inhibited migration of these highly metastatic cells. Additionally, anti-annexin II antibodies enhanced migration of these same cells in the wound closure assay, while an irrelevant antibody (anticalmodulin) showed no effect. These effects may be due to annexin-membrane binding and inhibition of phospholipid movement that is necessary for the formation of membrane protrusions. (C) 1997 Academic Press.