Short root mutant of Lotus japonicus with a dramatically altered symbiotic phenotype

被引:218
作者
Wopereis, J
Pajuelo, E
Dazzo, FB
Jiang, QY
Gresshoff, PM
de Bruijn, FJ
Stougaard, J
Szczyglowski, K
机构
[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
[2] Aarhus Univ, Dept Biol Mol & Struct, Gene Express Lab, DK-8000 Aarhus C, Denmark
[3] Michigan State Univ, Dept Microbiol, E Lansing, MI 48824 USA
[4] Univ Queensland, Dept Bot, Brisbane, Qld 4072, Australia
关键词
root development; nodulation; Rhizobium; phytohormones;
D O I
10.1046/j.1365-313x.2000.00799.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Legume plants carefully control the extent of nodulation in response to rhizobial infection. To examine the mechanism underlying this process we conducted a detailed analysis of the Lotus japonicus hypernodulating mutants, har1-1, 2 and 3 that define a new locus, HYPERNODULATION ABERRANT ROOT FORMATION (Har1), involved in root and symbiotic development. Mutations in the Har1 locus alter root architecture by inhibiting root elongation, diminishing root diameter and stimulating lateral root initiation. At the cellular level these developmental alterations are associated with changes in the position and duration of root cell growth and result in a premature differentiation of har1-1 mutant root. No significant differences between har1-1 mutant and wild-type plants were detected with respect to root growth responses to 1-aminocyclopropane1-carboxylic acid, the immediate precursor of ethylene, and auxin; however, cytokinin in the presence of AVG (aminoetoxyvinylglycine) was found to stimulate root elongation of the har1-1 mutant but not the wild-type. After inoculation with Mesorhizobium loti, the har1 mutant lines display an unusual hypernodulation (HNR) response, characterized by unrestricted nodulation (hypernodulation), and a concomitant drastic inhibition of root and shoot growth. These observations implicate a role for the Har1 locus in both symbiotic and non-symbiotic development of L. japonicus, and suggest that regulatory processes controlling nodule organogenesis and nodule number are integrated in an overall mechanism governing root growth and development.
引用
收藏
页码:97 / 114
页数:18
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