Nucleotide sequences of four pathogen-induced alfalfa peroxidase-encoding cDNAs

被引：16

作者：

ElTurk, J ^{[1
]}

Asemota, O ^{[1
]}

Leymarie, J ^{[1
]}

Sallaud, C ^{[1
]}

Mesnage, S ^{[1
]}

Breda, C ^{[1
]}

Buffard, D ^{[1
]}

Kondorosi, A ^{[1
]}

Esnault, R ^{[1
]}

机构：

[1] CNRS,INST SCI VEGETALES,F-91198 GIF SUR YVETTE,FRANCE

来源：

GENE | 1996年 / 170卷 / 02期

关键词：

Medicago sativa; Pseudomonas syringae; pathogen-induced defence reaction;

D O I：

10.1016/0378-1119(95)00830-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We constructed an alfalfa cDNA library from mRNA extracted from leaves after infection with Pseudomonas syringae (incompatible interaction). Screening with oligodeoxyribonucleotides designed from regions conserved in all known peroxidases allowed the isolation of four cDNAs (Mspsx1A, 1B, 1C and 2). Sequence analysis revealed the presence of open reading frames of 351, 355, 358 and 323 amino acids, respectively, with the characteristic consensus sequences of plant peroxidases. Sequence comparison showed that the Msprx2 product is significantly different from the others and, particularly, lacks a C-terminal propeptide which might be required for vacuolar targeting.

引用

页码：213 / 216

页数：4

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