Comparison of three selectable marker genes for transformation of wheat by microprojectile bombardment

被引：34

作者：

Witrzens, B ^{[1
]}

Brettell, RIS ^{[1
]}

Murray, FR ^{[1
]}

McElroy, D ^{[1
]}

Li, ZY ^{[1
]}

Dennis, ES ^{[1
]}

机构：

[1] CSIRO, Div Plant Ind, Canberra, ACT 2601, Australia

来源：

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY | 1998年 / 25卷 / 01期

关键词：

D O I：

10.1071/PP97095

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Three selectable marker genes were compared for their efficacy in the production of transgenic wheat plants following microprojectile bombardment of cultured immature embryos, While transformed plants were recovered using the bar (phosphinothricin acetyltransferase) gene in combination with bialaphos, and the aphA (neomycin phosphotransferase) gene in combination with geneticin or paromomycin, no transgenic material was obtained with the hpt (hygromycin phosphotransferase) gene and hygromycin B. Southern analysis revealed single copy as well as multiple copy insertions of the bar and aphA transgenes. Inheritance of these selectable marker genes was demonstrated in the T-1 generation progenies.

引用

页码：39 / 44

页数：6

共 33 条

[1] FERTILE TRANSGENIC WHEAT FROM MICROPROJECTILE BOMBARDMENT OF SCUTELLAR TISSUE [J].