Monocyte/macrophage β2-AR as a target of antisympathetic excitation-induced atherosclerotic progression

被引：10

作者：

Guo, Y. L. ^{[1
]}

Zhou, J. Q. ^{[1
]}

Xiang, C. Q. ^{[1
]}

Yang, W. H. ^{[1
]}

Zhang, B. ^{[1
]}

Dai, W. J. ^{[1
]}

Liu, J. H. ^{[1
]}

Zheng, S. J. ^{[1
]}

机构：

[1] Three Gorges Univ, Renhe Hosp, Cardiovasc Internal Dept, Yichang, Hubei, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2014年 / 13卷 / 04期

关键词：

beta; 2-AR; Mononuclear cell; Atherosclerosis; Stress; CORONARY HEART-DISEASE; BETA-BLOCKERS; MYOCARDIAL-INFARCTION; RISK; STRESS; WOMEN;

D O I：

10.4238/2014.October.7.2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The aim of this study was to determine whether monocyte/macrophage beta 2-AR could act as the therapeutic target of antisympathetic excitation-induced atherosclerotic progression. Cultivated human THP-1 cells were divided into different groups and incubated with isoprenaline, metoprolol, propranolol or beta 2-AR blocker for 24 h, together with oxidized low-density lipoprotein (ox-LDL). Afterwards, each group was analyzed for C-C chemokine receptor type 2 (CCR2) expression, monocyte chemotactic protein 1 (MCP-1) release into medium and cell migration ability. In the isoprenaline group, CCR2 protein level was increased, as well as the secretion of MCP-1, and cell motility was enhanced, in a concentration-dependent manner. Propranolol and ICI 118,551 significantly reversed the stimulatory effect of isoprenaline on THP-1 cells induced by ox-LDL, but only high concentrations of metoprolol interfered significantly with the action of isoprenaline (P < 0.05). Isoprenaline or a beta-AR blocker could mediate through beta 2-AR, affecting MCP-1 secretion, CCR2 protein expression and cell migration capacity of THP-1 cells. Therefore, monocyte-macrophage beta 2-AR may act as a target of antisympathetic excitation-induced atherosclerotic progression.

引用

页码：8080 / 8088

页数：9

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