Characterization of the Erwinia amylovora population in Israel

被引:14
作者
Manulis, S [1 ]
Kleitman, F
Dror, O
David, I
Zutra, D
机构
[1] Agr Res Org, Volcani Ctr, Dept Plant Pathol, IL-50250 Bet Dagan, Israel
[2] Agr Res Org, Volcani Ctr, Dept Fruit Trees, IL-50250 Bet Dagan, Israel
关键词
Erwinia amylovora; fire blight; RAPD analysis;
D O I
10.1007/BF02981264
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A collection of 205 strains of Erwinia amylovora isolated in Israel over a period of 12 years has been established. The strains were isolated from different varieties of pear, apple, loquat and quince grown in Israel, and collected from different locations in the country. They were characterized in respect to degree of virulence on several hosts and serological and molecular characters. Pathogenicity tests carried out on flowering branches of pear and apple, shoots of pears, and on trees of pear and loquat grown in containers outdoors, revealed no significant differences in the severity of blossom blight or shoot blight among the various strains. ELISA and immunofluorescence assays revealed nb serotypic groups among the Israeli strains. Genomic diversity was studied by random amplified polymorphic DNA (RAPD) analysis using 24 arbitrary 10-base primers. All the strains examined (45 Israeli and 11 from Egypt, Cyprus and Greece) produced the same RAPD patterns with each of the primers used. Amplification patterns were indistinguishable from those produced by strains isolated from the neighboring countries. Results presented in this study suggest that the population of E. amylovora in Israel is homogenous.
引用
收藏
页码:39 / 46
页数:8
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