Synthesis and evaluation of a 18F-labelled recombinant annexin-V derivative, for identification and quantification of apoptotic cells with PET

被引：92

作者：

Zijlstra, S ^{[1
]}

Gunawan, J ^{[1
]}

Burchert, W ^{[1
]}

机构：

[1] Univ Klinik Ruhr Bochum, Herz & Diabet Zentrum Nordrhein Westfalen, D-32545 Bad Oeynhausen, Germany

来源：

APPLIED RADIATION AND ISOTOPES | 2003年 / 58卷 / 02期

关键词：

D O I：

10.1016/S0969-8043(02)00302-0

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

In this report, we describe the synthesis of 4-[F-18]-fluorobenzoyl-annexin V (4-[F-18]FBA). In a four-step procedure, 4-[F-18]FBA was synthesised with a microcomputer controlled, automated module within 90 min. The radiochemical yield was in the range of 15-20% (corrected for decay) with a specific activity of more than 35 GBq/mumol. The specific binding was confirmed by studies of 4-[F-18]FBA with phosphatidylserine-containing liposomes. The biological activity of 4-[F-18]FBA was verified by measuring its binding to Jurkat T-cell lymphoblasts after induction of apoptosis as compared to control cells without apoptosis. 4-[F-18]FBA seems to be a suited tracer to measure apoptotic activity in vivo. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：201 / 207

页数：7

共 23 条

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