High level production of a cellulase-free xylanase in glucose-limited fed batch cultures of a thermophilic Bacillus strain

被引:28
作者
Samain, E [1 ]
Debeire, P [1 ]
Touzel, JP [1 ]
机构
[1] INRA,CPCB,UNITE PHYS CHIM & BIOTECHNOL POLYMERES,F-51687 REIMS 2,FRANCE
关键词
xylanase; Bacillus; enzymatic pulp bleaching; fed-batch;
D O I
10.1016/S0168-1656(97)00140-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacillus sp. strain XE and its mutant derivative strain D3 produce a thermostable xylanase which is suitable for enzymatic bleaching of kraft pulp. Xylanase synthesis was shown to be induced by the soluble products of xylan hydrolysis (xylooligosaccharide) and equally, catabolically-repressed when these oligosaccharides accumulate in the medium. An optimal balance between these two antagonistic effects was obtained in a carbon-limited, fed-batch culture with continuous xylooligosaccharide feeding. To reduce substrate cost, the xylooligosaccharide could be 90% substituted by glucose without reduction of the xylanase production rate. Xylanase production ceased when the activity reached approximately 380 U ml(-1) due to an amino acid shortage. A continuous supply of exogenous amino acids allowed the production to continue to more than 1000 U ml(-1). (C) 1997 Elsevier Science B.V.
引用
收藏
页码:71 / 78
页数:8
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