Pol κ:: A DNA polymerase required for sister chromatid cohesion

被引：160

作者：

Wang, ZH ^{[1
]}

Castano, IB ^{[1
]}

De Las Peñas, A ^{[1
]}

Adams, C ^{[1
]}

Christman, MF ^{[1
]}

机构：

[1] Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA

来源：

SCIENCE | 2000年 / 289卷 / 5480期

关键词：

D O I：

10.1126/science.289.5480.774

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Establishment of cohesion between sister chromatids is coupled to replication fork passage through an unknown mechanism. Here we report that TRF4, an evolutionarily conserved gene necessary for chromosome segregation, encodes a DNA polymerase with beta-polymerase-like properties. A double mutant in the redundant homologs, TRF4 and TRF5, is unable to complete S phase, whereas a trf4 single mutant completes a presumably defective S phase that results in a failure of cohesion between the replicated sister chromatids. This suggests that TRFs are a key Link in the coordination between DNA replication and sister chromatid cohesion. Trf4 and Trf5 represent the fourth class of essential nuclear DNA polymerases (designated DNA polymerase kappa) in Saccharomyces cerevisiae and probably in all eukaryotes.

引用

页码：774 / 779

页数：6

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