Isolation of Penicillium chrysogenum PEX1 and PEX6 encoding AAA proteins involved in peroxisome biogenesis

被引：32

作者：

Kiel, JAKW

Hilbrands, RE

Bovenberg, RAL

Veenhuis, M

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, NL-9751 NN Haren, Netherlands

[2] DSM Gist, DAI, PFG, NL-2600 MA Delft, Netherlands

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2000年 / 54卷 / 02期

关键词：

D O I：

10.1007/s002530000378

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In Penicillium chrysogenum, key enzymes involved in the production of penicillin reside in peroxisomes. As a first step to understand the role of these organelles in penicillin biosynthesis, we set out to isolate the genes involved in peroxisome biogenesis. Here we report the cloning and characterization of P. chrysogenum PEX1 and PEX6, which encode proteins of the AAA family of ATPases. The second AAA module, which is essential for the function of Pex1p and Pex6p in peroxisome biogenesis, is highly conserved in both PcPex1p and PcPex6p. PcPEX1 and PcPEX6 contain three and two introns, respectively.

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页码：238 / 242

页数：5

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