Measurement of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real-time polymerase chain reaction

被引：46

作者：

Yoneda, K

Chikumi, H

Murata, T

Gotoh, N

Yamamoto, H

Fujiwara, H

Nishino, T

Shimizu, E

机构：

[1] Tottori Univ, Fac Med, Dept Multidisciplinary Internal Med, Div Med Oncol & Mol Respirol, Tottori 6830805, Japan

[2] Kyoto Prefectural Univ, Dept Microbiol, Kyoto, Japan

[3] Tottori Univ Hosp, Dept Clin Lab, Yonago, Tottori 6730805, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 243卷 / 01期

关键词：

Pseudomonas aeruginosa; multidrug efflux pumps; real-time polymerase chain reaction; western blotting;

D O I：

10.1016/j.femsle.2004.11.048

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump protein quantities is unclear. We devised a real-time PCR for mRMA expression of MexAB-OprM and MexXY-OprM multidrug efflux pumps. In laboratory strains differing in MexB and MexY expression and in several clinical isolates, protein and mRNA expression correlated well. Quantitative real-time PCR should be a useful alternative in quantitating expression of multidrug efflux Pumps by P. aeruginosa isolates in clinical laboratories. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：125 / 131

页数：7

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