Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions

被引：390

作者：

Cheng, MY ^{[1
]}

Deng, JU ^{[1
]}

Yang, F ^{[1
]}

Gong, YD ^{[1
]}

Zhao, NM ^{[1
]}

Zhang, XF ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

来源：

BIOMATERIALS | 2003年 / 24卷 / 17期

关键词：

chitosan; gelatin; composite film; mechanical property; nerve cell affinity; nerve regeneration;

D O I：

10.1016/S0142-9612(03)00117-0

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A series of chitosan-gelatin composite films was prepared by varying the ratio of constituents. FT-IR and X-ray analysis showed good compatibility between these two biopolymers. Differential scanning calorimetry (DSC) analysis indicated that the water take-up of chitosan film increased when blended with gelatin. Composite film exhibited a lower Young's modulus and a higher percentage of elongation-at-break compared with chitosan film, especially in wet state. All composite films were hydrophilic materials with water contact angles ranging from 55degrees to 65degrees. The results obtained from ELISA indicated the adsorption amount of fibronectin oil composite films was much higher than on chitosan film. PC 12 cells culture was used to evaluate the nerve cell affinity of materials. The cells cultured on the composite film with 60wt% gelatin differentiated more rapidly and extended longer neurites than on chitosan film. The results suggest that the soft and elastic complex of chitosan and gelatin, which has better nerve cell affinity compared to chitosan, is a promising candidate biomaterial for nerve regeneration. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2871 / 2880

页数：10

共 29 条

[1] SYNTHETIC HYDROGELS .3. HYDROXYALKYL ACRYLATE AND METHACRYLATE COPOLYMERS - SURFACE AND MECHANICAL-PROPERTIES [J].