Specificity of a DNA-based (DNAzyme) peroxidative biocatalyst

被引：44

作者：

Rojas, Antonia M.

Gonzalez, Pedro A.

Antipov, Eugene

Klibanov, Alexander M. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 02期

关键词：

DNAzyme; hemin; peroxidase; regioselectivity; stereoselectivity;

D O I：

10.1007/s10529-006-9228-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A complex formation between hemin and a congruous oligonucleotide not only greatly enhances the former's peroxidative activity but also results in a biocatalyst (DNAzyme) with a novel specificity. Herein substrate, regio-, enantiomeric, and diastereomeric selectivities of heme, the DNAzyme, and the enzyme horseradish peroxidase are comparatively examined.

引用

页码：227 / 232

页数：6

共 13 条

[1]

Adam W, 1999, Adv Biochem Eng Biotechnol, V63, P73

[2] Molecular biology - Making catalytic DNAs [J].