R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells

被引：578

作者：

Yu, KF ^{[1
]}

Chedin, F ^{[1
]}

Hsieh, CL ^{[1
]}

Wilson, TE ^{[1
]}

Lieber, MR ^{[1
]}

机构：

[1] Univ So Calif, Keck Sch Med, Norris Comprehens Canc Ctr, Los Angeles, CA 90033 USA

来源：

NATURE IMMUNOLOGY | 2003年 / 4卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/ni919

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The mechanism responsible for immunoglobulin class switch recombination is unknown. Previous work has shown that class switch sequences have the unusual property of forming RNA-DNA hybrids when transcribed in vitro. Here we show that the RNA-DNA hybrid structure that forms in vitro is an R-loop with a displaced guanine (G)-rich strand that is single-stranded. This R-loop structure exists in vivo in B cells that have been stimulated to transcribe the gamma3 or the gamma2b switch region. The length of the R-loops can exceed I kilobase. We propose that this distinctive DNA structure is important in the class switch recombination mechanism

引用

页码：442 / 451

页数：10

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