Rapid purification and biochemical characteristics of lumbrokinase III from earthworm for use as a fibrinolytic agent

被引：26

作者：

Park, YD ^{[1
]}

Kim, JW ^{[1
]}

Min, BG ^{[1
]}

Seo, JW ^{[1
]}

Jeong, JM ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Med, Dept Biomed Engn, Seoul 151, South Korea

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 02期

关键词：

D O I：

10.1023/A:1005384625974

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fibrinolytic enzyme was purified from the earthworm (Lumbricus rubellus) by column chromatography and identified as lumbrokinase type III. Affinity chromatography and NH2-terminal amino acid sequences indicated that this lumbrokinase III-2 (34.2 kDa) had additional amino acids at the carboxyl terminus of lumbrokinase III-1 (34 kDa). The lumbrokinase III-1 was considerably stable at pH 2 to 11 and at up to 65 degrees C. It had trypsin-like characteristics with high substrate specificity against fibrin, suitable as a fibrinolytic agent. Degradation profiles of fibrinogen by lumbrokinase III-1 and their peptide sequences were also investigated.

引用

页码：169 / 172

页数：4

共 14 条

[1]

AHMED NK, 1990, HAEMOSTASIS, V20, P147

[2] PRECLINICAL PHARMACOLOGICAL EVALUATION OF ANISOYLATED PLASMINOGEN STREPTOKINASE ACTIVATOR COMPLEX [J].

FERRES, H .

DRUGS, 1987, 33 :33-50

[3] PLASMINOGEN ACTIVATORS AND THEIR POTENTIAL IN THERAPY [J].

HOLLANDER, IJ .

CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, 1987, 6 (03) :253-271

[4] DEGRADATION OF COAGULATION PROTEINS BY AN ENZYME FROM MALAYAN PIT VIPER (AKISTRODON-RHODOSTOMA) VENOM [J].

LOLLAR, P ;

PARKER, CG ;

KAJENSKI, PJ ;

LITWILLER, RD ;

FASS, DN .

BIOCHEMISTRY, 1987, 26 (24) :7627-7636

[5] Mechanism of thrombin inhibition by antithrombin and heparin cofactor II in the presence of heparin [J].

Maaroufi, RM ;

Jozefowicz, M ;

TaponBretaudiere, T ;

Fischer, AM .

BIOMATERIALS, 1997, 18 (03) :203-211

[6]

MIHARA H, 1983, THROMB HAEMOSTASIS, V50, P258

[7] A NOVEL FIBRINOLYTIC ENZYME EXTRACTED FROM THE EARTHWORM, LUMBRICUS-RUBELLUS [J].

MIHARA, H ;

SUMI, H ;

YONETA, T ;

MIZUMOTO, H ;

IKEDA, R ;

SEIKI, M ;

MARUYAMA, M .

JAPANESE JOURNAL OF PHYSIOLOGY, 1991, 41 (03) :461-472

[8] PURIFICATION, CHARACTERIZATION, AND FIBRINOGEN CLEAVAGE SITES OF 3 FIBRINOLYTIC ENZYMES FROM THE VENOM OF CROTALUS-BASILISCUS-BASILISCUS [J].

RETZIOS, AD ;

MARKLAND, FS .

BIOCHEMISTRY, 1992, 31 (19) :4547-4557

[9] FIBRINOLYTIC ENZYMES FROM THE VENOMS OF AGKISTRODON CONTORTRIX AND CROTALUS-BASILISCUS-BASILISCUS - CLEAVAGE SITE-SPECIFICITY TOWARDS THE ALPHA-CHAIN OF FIBRIN [J].

RETZIOS, AD ;

MARKLAND, FS .

THROMBOSIS RESEARCH, 1994, 74 (04) :355-367

[10] ANTITHROMBOGENICITY OF LUMBROKINASE-IMMOBILIZED POLYURETHANE [J].

RYU, GH ;

PARK, SY ;

KIM, M ;

HAN, DK ;

KIM, YH ;

MIN, BG .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1994, 28 (09) :1069-1077

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