Increased calpain expression is associated with apoptosis in rat spinal cord injury: Calpain inhibitor provides neuroprotection

被引：44

作者：

Ray, SK ^{[1
]}

Matzelle, DD ^{[1
]}

Wilford, GG ^{[1
]}

Hogan, EL ^{[1
]}

Banik, NL ^{[1
]}

机构：

[1] Med Univ S Carolina, Dept Neurol, Charleston, SC 29425 USA

来源：

NEUROCHEMICAL RESEARCH | 2000年 / 25卷 / 9-10期

关键词：

trauma; calpain; bax/bcl-2; ratio; apoptosis; E-64-d;

D O I：

10.1023/A:1007631826160

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calpain content was investigated in the lesion of rat spinal cord at 1, 4, 24, and 72 h following injury induced by the weight-drop (40 g-cm force) technique. Calpain content was increased in the lesion, and was highest at 24 h following injury. mu Calpain mRNA level in the lesion was increased by 58.4% (p = 0.0135) at 24 h following trauma, compared to sham. Alterations in mRNA expression in the lesion increased bax/bcl-2 ratio by 20.8% (p = 0.0395) at this time point, indicating a commitment to apoptosis. Therapeutic effect of the calpain inhibitor E-64-d (1 mg/kg) was studied in SCI rats following administration for 24 h. Internucleosomal DNA fragmentation (apoptosis) was observed in SCI rats, but not in sham or E-64-d treated rats. These results indicate a new information that E-64-d has the therapeutic potential for inhibiting apoptosis in SCI.

引用

页码：1191 / 1198

页数：8

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