Oxidation of 1-hydroxybenzotriazole by laccase and lignin peroxidase

被引：79

作者：

Ander, P ^{[1
]}

Messner, K ^{[1
]}

机构：

[1] Univ Technol Vienna, Dept Mycol, Inst Biochem Technol & Microbiol, A-1060 Vienna, Austria

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 03期

关键词：

D O I：

10.1023/A:1008813206178

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method to measure laccase and lignin peroxidase (LiP) activity at 408 nm (402-410 nm) using 1-hydroxybenzotriazole (HBT) was developed. The assay can be performed either as a kinetic measurement or as a stopped reaction using 5 mM Na-azide which improves the spectrum. Only white-rot fungal laccases and LiP were found to oxidize HBT to give shoulders or peaks at 402-410 nm. Phanerochaete and Phlebia manganese peroxidases did not give absorbance increase at 402-410 nm.

引用

页码：191 / 195

页数：5

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