Preparation, characterization and application of polyaniline nanospheres to biosensing

被引：79

作者：

Dhand, Chetna ^{[1
,2
]}

Das, Maumita ^{[1
,2
]}

Sumana, Gajjala ^{[1
]}

Srivastava, Avanish Kumar ^{[1
]}

Pandey, Manoj Kumar ^{[1
]}

Kim, Cheol Gi ^{[3
]}

Datta, Monika ^{[2
]}

Malhotra, Bansi Dhar ^{[1
,3
]}

机构：

[1] CSIR, Dept Sci & Technol, Ctr Biomol Elect, Natl Phys Lab, New Delhi 110012, India

[2] Univ Delhi, Dept Chem, Delhi 110007, India

[3] Chungnam Natl Univ, Ctr NanoBioEngn & Spintron, Taejon 305764, South Korea

来源：

NANOSCALE | 2010年 / 2卷 / 05期

关键词：

AMPHIPHILIC MOLECULAR DESIGN; CHOLESTEROL ESTERASE; NANOTUBES; NANOMATERIALS; NANOFIBERS; NANOWIRES; MEMBRANE; OXIDASE; FILMS; SIZE;

D O I：

10.1039/b9nr00346k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Polyaniline nanospheres (PANI-NS) prepared by morphological transformation of micelle polymerized camphorsulfonic acid (CSA) doped polyaniline nanotubes (PANI-NT) in the presence of ethylene glycol (EG) have been characterized by X-ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, Fourier transform infra-red and UV-Visible spectroscopy. A PANI-NS (60-80 nm) film deposited onto an indium-tin-oxide (ITO) coated glass plate by the solution casting method has been utilized for covalent immobilization of biomolecules (cholesterol oxidase (ChOx)) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry for fabrication of a cholesterol biosensor. The ChOx/PANI-NS/ITO bioelectrode detects cholesterol in the concentration range of 25 to 500 mg dL(-1) with sensitivity of 1.3 x 10(-3) mA mg(-1) dL and regression coefficient of 0.98. Further, this PANI-NS based bioelectrode shows fast response time (10 s), low Michaelis-Menten constant (2.5 mM) and shelf-life of 12 weeks. The spherical nanostructure observed in the final morphology of the PANI-NS film is attributed to hydrogen bonding interactions between PANI-NT and EG.

引用

页码：747 / 754

页数：8

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