Comparison of neutralising antibody assays for detection of antibody to influenza A/H3N2 viruses: An international collaborative study

被引:95
作者
Stephenson, Iain [1 ]
Das, Rose Gaines
Wood, John M.
Katz, Jacqueline M.
机构
[1] Univ Hosp Leicester, Infect Dis Unit, Leicester LE1 5WW, Leics, England
[2] Natl Inst Biol Stand & Controls, Potters Bar EN6 3QG, Herts, England
[3] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA USA
基金
英国医学研究理事会;
关键词
influenza; serology; assays;
D O I
10.1016/j.vaccine.2007.02.039
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A study was performed to investigate the reproducibility of haemagglutinin-inhibition (111) and virus neutralising (VN) assays for detection of anti-influenza antibody. Participants in I I laboratories from eight countries measured antibody to ego-grown A/Japan/434/2003, cell-grown A/Japan/434/2003 and A/Panama/2007/99 (H3N2) viruses in 18 human and two post-infection ferret sera. There was significant intra-laboratory assay variability for VN compared to HI. For replicate assays within laboratories, 14/410 (3%) and 130/631 (21%) fitres differed by > 2-fold (p < 0.0001), and 0/410 (0%) and 35/631 (6%) titres differed by > 5-fold (p < 0.0001) by HI and VN, respectively. Although both assays showed inter-laboratory variation, VN assays were significantly more variable than III. Median geometric coefficients of variation (GCV) for VN assays with each virus were 256%, 323% and 359% compared to 138%, 155% and 261% with HI. A serum standard improved inter-laboratory agreement and reduced median GCVs. This study raises concern about comparability of serology results from H5N1 vaccine trials and it is proposed that an International Standard for influenza H5N1 antibody is developed. Crown Copyright (c) 2007 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:4056 / 4063
页数:8
相关论文
共 17 条
[1]   REPLICATION OF AVIAN INFLUENZA-VIRUSES IN HUMANS [J].
BEARE, AS ;
WEBSTER, RG .
ARCHIVES OF VIROLOGY, 1991, 119 (1-2) :37-42
[2]   NEUTRALIZATION ENZYME-IMMUNOASSAY FOR INFLUENZA-VIRUS [J].
BENNE, CA ;
HARMSEN, M ;
DEJONG, JC ;
KRAAIJEVELD, CA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (04) :987-990
[3]   Safety and immunogenicity of an inactivated split-virion influenza A/Vietnam/1194/2004 (H5N1) vaccine:: phase I randomised trial [J].
Bresson, Jean-Louis ;
Perronne, Christian ;
Launay, Odile ;
Gerdil, Catherine ;
Saville, Melanie ;
Wood, John ;
Hoeschler, Katja ;
Zambon, Maria C. .
LANCET, 2006, 367 (9523) :1657-1664
[4]   Report of a collaborative study to calibrate the Second International Standard for parvovirus B19 antibody [J].
Ferguson, M ;
Heath, A .
BIOLOGICALS, 2004, 32 (04) :207-212
[5]   MICRONEUTRALIZATION TEST FOR INFLUENZA-A AND INFLUENZA-B AND PARA-INFLUENZA-1 AND PARAINFLUENZA-2 VIRUSES THAT USES CONTINUOUS CELL-LINES AND FRESH SERUM ENHANCEMENT [J].
FRANK, AL ;
PUCK, J ;
HUGHES, BJ ;
CATE, TR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1980, 12 (03) :426-432
[6]   ROLE OF SERUM HEMAGGLUTINATION-INHIBITING ANTIBODY IN PROTECTION AGAINST CHALLENGE INFECTION WITH INFLUENZA A2 AND B VIRUSES [J].
HOBSON, D ;
CURRY, RL ;
BEARE, AS ;
WARDGARD.A .
JOURNAL OF HYGIENE, 1972, 70 (04) :767-777
[7]   Safety and immunogenicity of an inactivated adjuvanted whole-virion influenza A (H5N1) vaccine: a phase I randomised controlled trial [J].
Lin, Jiangtao ;
Zhang, Jiansan ;
Dong, Xiaoping ;
Fang, Hanhua ;
Chen, Jiangting ;
Su, Nan ;
Gao, Qiang ;
Zhang, Zhenshan ;
Liu, Yuxuan ;
Wang, Zhihong ;
Yang, Meng ;
Sun, Ruihua ;
Li, Changgui ;
Lin, Su ;
Ji, Mei ;
Liu, Yan ;
Wang, Xu ;
Wood, John ;
Feng, Zijian ;
Wang, Yu ;
Yin, Weidong .
LANCET, 2006, 368 (9540) :991-997
[8]   Safety and antigenicity of non-adjuvanted and MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine: a randomised trial of two potential vaccines against H5N1 influenza [J].
Nicholson, KG ;
Colegate, AE ;
Podda, A ;
Stephenson, I ;
Wood, J ;
Ypma, E ;
Zambon, MC .
LANCET, 2001, 357 (9272) :1937-1943
[9]   RAPID FOCUS REDUCTION NEUTRALIZATION TEST OF INFLUENZA-A AND INFLUENZA-B VIRUSES IN MICROTITER SYSTEM [J].
OKUNO, Y ;
TANAKA, K ;
BABA, K ;
MAEDA, A ;
KUNITA, N ;
UEDA, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (06) :1308-1313
[10]   DETERMINANTS OF IMMUNITY TO INFLUENZA INFECTION IN MAN [J].
POTTER, CW ;
OXFORD, JS .
BRITISH MEDICAL BULLETIN, 1979, 35 (01) :69-75