Identification of AFLP fragments linked to one recessive genic male sterility (RGMS) in rapeseed (Brassica napus L.) and conversion to SCAR markers for marker-aided selection

被引:28
作者
Ke, LP [1 ]
Sun, YQ [1 ]
Liu, PW [1 ]
Yang, GS [1 ]
机构
[1] Huazhong Agr Univ, Natl Ctr Rapeseed Improvement Wuhan, NAtl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
基金
中国国家自然科学基金;
关键词
AFLP; Brassica napus; Recessive genic male sterility gene (ms); SCAR;
D O I
10.1023/B:EUPH.0000046800.29308.b0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
117AB is a recessive genic male sterility (RGMS) line in which the sterility is controlled by a duplicate recessive gene named ms, located at two separate loci. In the RGMS line, the genotype of the sterile plant (117A) is msmsmsms, and that of the fertile plant (117B) is Msmsmsms. The present study was aimed to identify DNA markers linked to the ms locus by amplified fragment length polymorphism (AFLP). From the survey of 512 AFLP primer combinations, 6 AFLP fragments (y1, k1, k2, k3, k4, k5) were identified as being tightly linked to the Ms locus. The genetic distances between the markers and the Ms locus were all less than 8 cM, among which two fragments, designated as k2 and k3, co-segregated with the target gene in the tested population. Fragment k2 was successfully converted into a sequence characterized amplified region ( SCAR) marker. The markers detected could be valuable in marker-assisted breeding of RGMS in Brassica napus.
引用
收藏
页码:163 / 168
页数:6
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