A reinvestigation of sulfenyl groups as amino protecting groups for the synthesis of oligonucleotides on solid support by phosphoramidite chemistry

被引：9

作者：

Alvarez, K ^{[1
]}

Tworkowski, I ^{[1
]}

Vasseur, JJ ^{[1
]}

Imbach, JL ^{[1
]}

Rayner, B ^{[1
]}

机构：

[1] Univ Montpellier 2, Chim Bioorgan Lab, CNRS, UMR 5625,UM II, F-34095 Montpellier 5, France

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1998年 / 17卷 / 1-3期

关键词：

D O I：

10.1080/07328319808005184

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although sulfenyl groups as protectors of heterocyclic amines of nucleosides appeared satisfactory during the synthesis of DNA and RNA via the phosphotriester approach, their usefulness in automated synthesis of oligonucleotides using phosphoramidite chemistry has not been investigated. Herein, we examined the stability and efficiency of 2-nitrophenylsulfenyl- and tritylsulfenyl-nucleosides upon the conditions applied in oligonucleotide synthesis by the phosphoramidite approach.

引用

页码：365 / 378

页数：14

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