Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase

被引:269
作者
Richins, RD [1 ]
Kaneva, I [1 ]
Mulchandani, A [1 ]
Chen, W [1 ]
机构
[1] UNIV CALIF RIVERSIDE, DEPT CHEM ENGN, RIVERSIDE, CA 92521 USA
关键词
bioremediation; parathion; immobilized bioreactor;
D O I
10.1038/nbt1097-984
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. inclusion of the organophosphorus hydrolase signal sequence was necessary for achieving enzymatic activity on the surface. More than 80% of the OPH activity was located on the cell surface as determined by protease accessibility experiments. Whole cells expressing OPH on the cell surface degraded parathion and paraoxon very effectively without any diffusional limitation, resulting in sevenfold higher rates of parathion degradation compared with whole cells with similar levels of intracellular OPH. Immobilization of these live biocatalysts onto solid supports could provide an attractive means for pesticide detoxification in place of immobilized enzymes, affording a reduced diffusional barrier.
引用
收藏
页码:984 / 987
页数:4
相关论文
共 28 条
  • [1] BACTERIAL DETOXIFICATION OF DIISOPROPYL FLUOROPHOSPHATE
    ATTAWAY, H
    NELSON, JO
    BAYA, AM
    VOLL, MJ
    WHITE, WE
    GRIMES, DJ
    COLWELL, RR
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (07) : 1685 - 1689
  • [2] STRUCTURE AND MORPHOLOGY OF PROTEIN INCLUSION-BODIES IN ESCHERICHIA-COLI
    BOWDEN, GA
    PAREDES, AM
    GEORGIOU, G
    [J]. BIO-TECHNOLOGY, 1991, 9 (08): : 725 - 730
  • [3] REGULATION OF THE ESCHERICHIA-COLI HEAT-SHOCK RESPONSE
    BUKAU, B
    [J]. MOLECULAR MICROBIOLOGY, 1993, 9 (04) : 671 - 680
  • [4] DETOXIFICATION OF ORGANOPHOSPHATE PESTICIDES USING AN IMMOBILIZED PHOSPHOTRIESTERASE FROM PSEUDOMONAS-DIMINUTA
    CALDWELL, SR
    RAUSHEL, FM
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (02) : 103 - 109
  • [5] The expression of a plasmid-specified exported protein causes structural plasmid instability in Bacillus subtilis
    Cordes, C
    Meima, R
    Twiest, B
    Kazemier, B
    Venema, G
    vanDijl, JM
    Bron, S
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (17) : 5235 - 5242
  • [6] ISOLATION OF COVALENTLY CLOSED CIRCULAR DNA OF HIGH MOLECULAR-WEIGHT FROM BACTERIA
    CURRIER, TC
    NESTER, EW
    [J]. ANALYTICAL BIOCHEMISTRY, 1976, 76 (02) : 431 - 441
  • [7] CHARACTERIZATION OF ORGANOPHOSPHORUS HYDROLASES AND THE GENETIC MANIPULATION OF THE PHOSPHOTRIESTERASE FROM PSEUDOMONAS-DIMINUTA
    DAVE, KI
    MILLER, CE
    WILD, JR
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1993, 87 (1-3) : 55 - 68
  • [8] DUMAS DP, 1989, BIOTECHNOL APPL BIOC, V11, P235
  • [9] DUMAS DP, 1989, J BIOL CHEM, V264, P19659
  • [10] INACTIVATION OF ORGANOPHOSPHORUS NERVE AGENTS BY THE PHOSPHOTRIESTERASE FROM PSEUDOMONAS-DIMINUTA
    DUMAS, DP
    DURST, HD
    LANDIS, WG
    RAUSHEL, FM
    WILD, JR
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 277 (01) : 155 - 159