Efficiency of mitochondrial DNA restriction analysis and RAPD-PCR to characterize yeasts growing on dry-cured Iberian ham at the different geographic areas of ripening

被引：10

作者：

Andrade, Maria J. ^{[1
]}

Rodriguez, Mar ^{[1
]}

Casado, Eva ^{[1
]}

Cordoba, Juan J. ^{[1
]}

机构：

[1] Univ Extremadura, Fac Vet, Caceres 10071, Spain

来源：

MEAT SCIENCE | 2010年 / 84卷 / 03期

关键词：

Yeasts; Dry-cured Iberian ham; Molecular characterization; SACCHAROMYCES-CEREVISIAE STRAINS; MOLECULAR-IDENTIFICATION; DEBARYOMYCES-HANSENII; VOLATILE COMPOUNDS; GENETIC DIVERSITY; DAIRY-PRODUCTS; WINE; POPULATION; SOURDOUGHS; SEQUENCES;

D O I：

10.1016/j.meatsci.2009.09.005

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The efficiency of mitochondrial DNA (mtDNA) restriction analysis and random amplification of polymorphic DNA (RAPD)-PCR to characterize yeasts growing on dry-cured Iberian ham was evaluated. Besides. the distribution of the main species and biotypes of yeasts in the different ripening areas of this product was investigated. MtDNA restriction analysis allowed yeast characterization at species and strain level. RAPD-PCR with the primers (GACA)(4) and (GAC)(5) was inappropriate for characterization at species level. Most of the mtDNA restriction patterns detected in dry-cured Iberian ham were consistent with Debaryomyces hansenii. Several yeasts biotypes were associated to specific geographic areas of dry-cured Iberian ham ripening. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：377 / 383

页数：7

共 36 条

[1] DNA typing methods for differentiation of yeasts related to dry-cured meat products [J].