Functionalization of PVC membrane with ss oligonucleotides for a potentiometric biosensor

被引：24

作者：

Shishkanova, T. V. ^{[1
]}

Volf, R. ^{[1
]}

Krondak, M. ^{[1
]}

Kral, V. ^{[1
]}

机构：

[1] Inst Chem Technol, Dept Analyt Chem, CR-16628 Prague 6, Czech Republic

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 11期

关键词：

ion-selective electrode; oligonucleotide; immobilization; hybridization;

D O I：

10.1016/j.bios.2006.11.014

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)(15), modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)15 on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA(+)Cl(-)). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)(15) modified membranes were sensitive toward complementary oligo(dT)(15) in the concentration range 2-80 nM at pH 7. An explanation for the detection mechanism is proposed. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：2712 / 2717

页数：6

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