Imaging of ultra-weak bio-chemiluminescence and singlet oxygen generation in germinating soybean in response to wounding

被引：35

作者：

Chen, WL ^{[1
]}

Xing, D ^{[1
]}

Tan, SC ^{[1
]}

Tang, YH ^{[1
]}

He, YH ^{[1
]}

机构：

[1] S China Normal Univ, Laser Life Sci Inst, Guangzhou 510631, Peoples R China

来源：

LUMINESCENCE | 2003年 / 18卷 / 01期

关键词：

ultra-weak bio-chemiluminescence; soybean cotyledon; wounding; defence response; singlet oxygen;

D O I：

10.1002/bio.703

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ultra-weak bio-chemiluminescence (UBC) from germinating soybean (Glycine max L. Merr) cotyledon under mechanical wounding was observed using a high-sensitivity imaging system based on an ICCD detector and a highly sensitive single photon counter (SPC) device. The UBC imaging showed that the intensity at the injury location on a wounded cotyledon was obviously enhanced as compared with that at the non-injured point. The UBC intensity of wounded cotyledons was initially very high and reached a stationary state after about 5 min. Wounding-induced emission could be suppressed by wounding in the presence of sodium azide. Deuterium oxide amplified the emission intensity. It was concluded that singlet oxygen (102) was the main cause of the emission during the wounding phase. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：37 / 41

页数：5

共 21 条

[1] EVIDENCE FOR GENERATION OF AN ELECTRONIC EXCITATIONS STATES) IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND ITS PARTICIPATION IN BACTERICIDAL ACTIVITY [J].

ALLEN, RC ;

STEELE, RH ;

STJERNHOLM, RL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 47 (04) :679-+

[2]

AVELES BF, 1978, PLANT PHYSIOL, V62, P696

[3] Mechanisms for the generation of reactive oxygen species in plant defence - a broad perspective [J].

Bolwell, GP ;

Wojtaszek, P .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1997, 51 (06) :347-366

[4]

CAMP WV, 1997, FREE RADIC BIOL MED, V23, P151

[5] The Pto kinase mediates a signaling pathway leading to the oxidative burst in tomato [J].

Chandra, S ;

Martin, GB ;

Low, PS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (23) :13393-13397

[6]

CHEN WL, 2002, IN PRESS LUMINESCENC, V17

[7] Dual action of the active oxygen species during plant stress responses [J].

Dat, J ;

Vandenabeele, S ;

Vranová, E ;

Van Montagu, M ;

Inzé, D ;

Van Breusegem, F .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2000, 57 (05) :779-795

[8] DARK ADAPTED LEAVES OF PARAQUAT-RESISTANT TOBACCO PLANTS EMIT LESS ULTRAWEAK LIGHT THAN SUSCEPTIBLE ONES [J].

HIDEG, E ;

INABA, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 178 (02) :438-443

[9] TWO-DIMENSIONAL IMAGING OF ULTRA-WEAK EMISSION FROM INTACT SOYBEAN ROOTS [J].

ICHIMURA, T ;

HIRAMATSU, M ;

HIRAI, N ;

HAYAKAWA, T .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1989, 50 (03) :283-286

[10] Ultraweak luminescence generated by sweet potato and Fusarium oxysporum interactions associated with a defense response [J].

Makino, T ;

Kato, K ;

Iyozumi, H ;

Honzawa, H ;

Tachiiri, Y ;

Hiramatsu, M .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1996, 64 (06) :953-956

← 1 2 3 →