Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells

被引：40

作者：

Roche, RR ^{[1
]}

Alvarez, M ^{[1
]}

Guzmán, MG ^{[1
]}

Morier, L ^{[1
]}

Kourí, G ^{[1
]}

机构：

[1] Pedro Kouri Trop Med Inst, Dept Virol, PAHO, WHO Collaborator Ctr Viral Dis, Havana, Cuba

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 09期

关键词：

D O I：

10.1128/JCM.38.9.3508-3510.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.

引用

页码：3508 / 3510

页数：3

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