Determination of carbamate residues in crop samples by cholinesterase-based biosensors and chromatographic techniques

被引:77
作者
Nunes, GS
Skládal, P
Yamanaka, H
Barceló, D
机构
[1] UFMA, Dept Chem Technol, BR-65080040 Sao Luis, MA, Brazil
[2] Masaryk Univ, Dept Biochem, Brno 261137, Czech Republic
[3] UNESP, Inst Chem, Dept Analyt Chem, BR-14900040 Araraquara, SP, Brazil
[4] CSIC, Cid, Dept Environm Chem, Barcelona 08040, Spain
关键词
carbamates; acetylcholinesterases; crop samples; amperometric biosensors;
D O I
10.1016/S0003-2670(97)00547-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An amperometric biosensor based on cholinesterase (ChE) has been used for the determination of selected carbamate insecticides in vegetable samples. The linear range of the biosensor for the N-methylcarbamates (aldicarb, carbaryl, carbofuran, methomyl and propoxur) varied from 5 x 10(-5) to 50 mg kg(-1). Limits of detection were calculated on the basis that the ChE enzymes were 10% inhibited and varied, depending of the combination ChE (as acetyl- or butyrylcholinesterase) vs. inhibitor (pesticide), from 1 x 10(-4) to 3.5 mg kg(-1). The biosensor-based carbamate determination was compared to liquid chromatography/UV methods. Three vegetable samples were spiked with carbofuran and propoxur at 125 mu g kg(-1) followed by conventional procedures. Good correlations were observed for carbofuran in the vegetable extracts (79, 96 and 91% recoveries for potato, carrot and sweet pepper, respectively), whereas for propoxur unsatisfactory results were obtained. Potato and carrot samples were spiked with 10, 50 and 125 mu g kg(-1) carbofuran, followed by direct determination by the amperometric biosensor. The fortified sampler; resulted in very high inhibition values, and recoveries were: 28, 34 and 99% for potato, and 140, 90 and 101% for carrot, respectively, at these three fortification levels. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:59 / 68
页数:10
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