Yeast Microflora Isolated From Brazilian Cassava Roots: Taxonomical Classification Based on Molecular Identification

被引：27

作者：

Ferreira, Nelson ^{[1
]}

Belloch, Carmela ^{[2
]}

Querol, Amparo ^{[2
]}

Manzanares, Paloma ^{[2
]}

Vallez, Salvador ^{[2
]}

Santos, Alberdan ^{[1
]}

机构：

[1] Fed Univ Para, Lab Invest Sistemat Biotecnol & Quim Fina, BR-66059 Belem, Para, Brazil

[2] CSIC, Inst Agroquim & Tecnol Alimentos, E-46010 Valencia, Spain

来源：

CURRENT MICROBIOLOGY | 2010年 / 60卷 / 04期

关键词：

ASCOMYCETOUS YEASTS; RIBOSOMAL-RNA; DNA; PHYLOGENY; DIVERSITY; 18S;

D O I：

10.1007/s00284-009-9539-z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid molecular identification technique was applied on microbial microflora isolated from Brazilian cassava roots given a yeast profile presented in the samples analyzed. A total of 24 strain isolated from cassava were initially grouped and identified in five groups using restriction-fragment length polymorphism (RFLPs) of 5.8S-ITS rDNA region. Sequencing analysis of the domains D1 and D2 of the 26S rRNA gene or 5.8S rRNA-ITS region were used to identify different groups of yeasts. Representative colonies of yeasts of each group were isolated and identified as Debaromyces hansenii, Kodamaea ohmeri, Candida glabrata, C. haemulonii, and Pichia gullhermondii. It is hoped that these results will contribute toward selecting yeast from this microflora capable to degrade cassava starch in the near future.

引用

页码：287 / 293

页数：7

共 22 条

[1] Microbiological characterization of yam fermentation for 'Elubo' (yam flour) production [J].

Achi, OK ;

Akubor, PI .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2000, 16 (01) :3-7

[2]

Alves Alfredo Augusto Cunha, 2001, P67, DOI 10.1079/9780851995243.0067

[3]

Dixon A. G. O., 2000, African Crop Science Journal, V8, P1

[4] RESPONSE OF CASSAVA TO WATER-STRESS [J].

ELSHARKAWY, MA ;

COCK, JH .

PLANT AND SOIL, 1987, 100 (1-3) :345-360

[5] Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers [J].

Esteve-Zarzoso, B ;

Belloch, C ;

Uruburu, F ;

Querol, A .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :329-337

[6] Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR [J].

Fredricks, DN ;

Smith, C ;

Meier, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) :5122-5128

[7] Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene [J].

Kurtzman, CP ;

Robnett, CJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (05) :1216-1223

[8] Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences [J].

Kurtzman, CP ;

Robnett, CJ .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1998, 73 (04) :331-371

[9] Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions [J].

Leaw, SN ;

Chang, HC ;

Sun, HF ;

Barton, R ;

Bouchara, JP ;

Chang, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (03) :693-699

[10]

Liebmann B, 2001, J CLIMATE, V14, P4308, DOI 10.1175/1520-0442(2001)014<4308:IVOTRS>2.0.CO

← 1 2 3 →