Iron-coproporphyrin III is a natural cofactor in bacterioferritin from the anaerobic bacterium Desulfovibrio desulfuricans

被引：29

作者：

Romao, CV

Louro, R

Timkovich, R

Lübben, M

Liu, MY

LeGall, J

Xavier, AV

Teixeira, M

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780156 Oeiras, Portugal

[2] Univ Alabama, Dept Chem, Tuscaloosa, AL 35487 USA

[3] Ruhr Univ Bochum, Lehrstuhl Biophys, D-44780 Bochum, Germany

[4] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

来源：

FEBS LETTERS | 2000年 / 480卷 / 2-3期

关键词：

bacterioferritin; coproporphyrin; heme biosynthesis; Desulfovibrio;

D O I：

10.1016/S0014-5793(00)01939-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A bacterioferritin was recently isolated from the anaerobic sulphate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 [Romao et al, (2000) Biochemistry 39, 6841-6849]. Although its properties are in general similar to those of the other bacterioferritins, it contains a haem quite distinct from the haem B, found in bacterioferritins from aerobic organisms. Using visible and NMR spectroscopies, as well as mass spectrometry analysis, the haem is now unambiguously identified as iron-coproporphyrin III, the first example of such a prosthetic group in a biological system, This unexpected finding is discussed in the framework of haem biosynthetic pathways in anaerobes and particularly in sulphate-reducing bacteria. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved.

引用

页码：213 / 216

页数：4

共 30 条

[1] L-METHIONINE METHYL IS SPECIFICALLY INCORPORATED INTO THE C-2 AND C-7 POSITIONS OF THE PORPHYRIN OF CYTOCHROME-C(3) IN A STRICTLY ANAEROBIC BACTERIUM, DESULFOVIBRIO-VULGARIS [J].