A simple method for the eradication of Legionella pneumophila from potable water systems

被引:5
作者
Moreno, C [1 ]
de Blas, I [1 ]
Miralles, F [1 ]
Apraiz, D [1 ]
Catalan, V [1 ]
机构
[1] Labaqua SA, Alicante 03007, Spain
关键词
Legionella pneumophila; eradication;
D O I
10.1139/m97-168
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this paper we describe a simple method, noncorrosive to pipes, for the eradication of Legionella pneumophila from potable water systems. This method is based on the systematic purging of the pipe networks with cold water containing 1-1.5 mg residual chlorine/L. In the hot water system, a new pipe bypassing the water heater was installed, whereas in the air conditioning system, the circuit is Purged with water from the tap water system. The feasibility of this method was studied in two hotels in which the presence of Legionella was detected despite treatment of the water by the hyperchlorination method. The evolution of the presence of Legionella was studied by culture and polymerase chain reaction. Eighty samples from hotel A and sixty-seven samples from hotel B were analyzed during the time that the eradication method was applied. Our results showed that this method permitted the effective elimination of L. pneumophila after 5 months in hotel A and 7 months in hotel B.
引用
收藏
页码:1189 / 1196
页数:8
相关论文
共 49 条
[1]   DETECTION OF ENTEROVIRUSES IN GROUNDWATER WITH THE POLYMERASE CHAIN-REACTION [J].
ABBASZADEGAN, M ;
HUBER, MS ;
GERBA, CP ;
PEPPER, IL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (05) :1318-1324
[2]  
[Anonymous], [No title captured]
[3]   SUSCEPTIBILITY OF LEGIONELLA-PNEUMOPHILA TO ULTRAVIOLET-RADIATION [J].
ANTOPOL, SC ;
ELLNER, PD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1979, 38 (02) :347-348
[4]   PROTOCOL FOR SAMPLING ENVIRONMENTAL SITES FOR LEGIONELLAE [J].
BARBAREE, JM ;
GORMAN, GW ;
MARTIN, WT ;
FIELDS, BS ;
MORRILL, WE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (07) :1454-1458
[5]   DETECTION OF VIABLE LEGIONELLA-PNEUMOPHILA IN WATER BY POLYMERASE CHAIN-REACTION AND GENE PROBE METHODS [J].
BEJ, AK ;
MAHBUBANI, MH ;
ATLAS, RM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (02) :597-600
[6]  
BEST M, 1984, AM J INFECT CONTROL, V12, P1226
[7]   ISOLATION OF LEGIONELLA SPP FROM ENVIRONMENTAL WATER SAMPLES BY LOW-PH TREATMENT AND USE OF A SELECTIVE MEDIUM [J].
BOPP, CA ;
SUMNER, JW ;
MORRIS, GK ;
WELLS, JG .
JOURNAL OF CLINICAL MICROBIOLOGY, 1981, 13 (04) :714-719
[8]   Detection of Legionella pneumophila in wastewater by nested polymerase chain reaction [J].
Catalan, V ;
Garcia, F ;
Moreno, C ;
Vila, MJ ;
Apraiz, D .
RESEARCH IN MICROBIOLOGY, 1997, 148 (01) :71-78
[9]   NESTED POLYMERASE CHAIN-REACTION FOR DETECTION OF LEGIONELLA-PNEUMOPHILA IN WATER [J].
CATALAN, V ;
MORENO, C ;
DASI, MA ;
MUNOZ, C ;
APRAIZ, D .
RESEARCH IN MICROBIOLOGY, 1994, 145 (08) :603-610
[10]  
Clesceri L.S., 1989, STANDARD METHODS EXA, V17th