Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography

被引：14

作者：

Davey, MW

Persiau, G

De Bruyn, A

Van Damme, S

Bauw, G

Van Montagu, M

机构：

[1] State Univ Ghent VIB, Dept Genet, Genet Lab, B-9000 Ghent, Belgium

[2] Univ Ghent, Vakgrp Org Scheikunde, B-9000 Ghent, Belgium

[3] State Univ Ghent VIB, Lab Fysiol Scheikunde, B-9000 Ghent, Belgium

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 257卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2544

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The pyrrolophenanthridine alkaloid lycorine has frequently been used as a specific inhibitor to help elucidate the function of ascorbic acid (vitamin C) in a wide range of biological processes. It was recently reported that this function can be exercised by inhibiting the activity of L-galactono-1,4-lactone dehydrogenase, the terminal enzyme of ascorbic acid biosynthesis, although working with the purified enzyme, we have been unable to repeat this result. Here, we present a procedure for the purification and analysis of lycorine by high-performance liquid chromatography from two Crinum species and describe for the first time a method that allows the simultaneous analysis of ascorbic acid and lycorine in tissue extracts of Crinum asiaticum by micellar electrokinetic chromatography.

引用

页码：80 / 88

页数：9

共 40 条

[1]

THE ALKALOIDS OF CRINUM-AUGUSTUM .1. 2 NON-HYDROXYLATED ALKALOIDS IN CRINUM-AUGUSTUM [J].