Validated high-performance liquid chromatographic method for the determination of lamotrigine in human plasma

被引：49

作者：

Barbosa, NR ^{[1
]}

Mídio, AF ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 Sao Paulo, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 741卷 / 02期

关键词：

lamotrigine;

D O I：

10.1016/S0378-4347(00)00102-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) procedure for lamotrigine was developed and validated. Lamotrigine (LTG) and an internal standard were extracted from plasma using liquid-liquid extraction under alkaline conditions into an organic solvent. The method was linear in the range 0.78-46.95 mu mol/l, with a mean coefficient of correlation (r)greater than or equal to 0.99923. The limit of detection (LOD) and limit of quantification (LOQ) were 0.19 and 0.58 mu mol/l, respectively. Within- and between-run precision studies demonstrated C.V.<3% at all tested concentrations. LTG median recovery was 86.14%. Antiepileptic drugs tested did not interfere with the assay. The method showed to be appropriate for monitoring LTG in plasma samples. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：289 / 293

页数：5

共 24 条

[1] Determination of lamotrigine in human plasma by high-performance liquid chromatography [J].

Angelis-Stoforidis, P ;

Morgan, DJ ;

O'Brien, TJ ;

Vajda, FJE .

JOURNAL OF CHROMATOGRAPHY B, 1999, 727 (1-2) :113-118

[2] Lamotrigine drug interactions in a TDM material [J].

Böttiger, Y ;

Svensson, JO ;

Ståhle, L .

THERAPEUTIC DRUG MONITORING, 1999, 21 (02) :171-174

[3]

BOUTAGY J, 1995, THER DRUG MONIT, V17, P410

[4] NEW AND POTENTIAL ANTICONVULSANTS [J].

BRODIE, MJ ;

PORTER, RJ .

LANCET, 1990, 336 (8712) :425-426

[5] LAMOTRIGINE [J].

BRODIE, MJ .

LANCET, 1992, 339 (8806) :1397-1400

[6] PERFORMANCE ANALYSIS OF A REVERSED-PHASE LIQUID-CHROMATOGRAPHIC ASSAY OF LAMOTRIGINE IN PLASMA USING SOLVENT-DEMIXING EXTRACTION [J].

COCIGLIO, M ;

ALRIC, R ;

BOUVIER, O .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 572 (1-2) :269-276

[7] LAMOTRIGINE, A NEW ANTICONVULSANT - PHARMACOKINETICS IN NORMAL HUMANS [J].

COHEN, AF ;

LAND, GS ;

BREIMER, DD ;

YUEN, WC ;

WINTON, C ;

PECK, AW .

CLINICAL PHARMACOLOGY & THERAPEUTICS, 1987, 42 (05) :535-541

[8] A LIQUID-CHROMATOGRAPHIC ASSAY USING A HIGH-SPEED COLUMN FOR THE DETERMINATION OF LAMOTRIGINE, A NEW ANTIEPILEPTIC DRUG, IN HUMAN PLASMA [J].

FAZIO, A ;

ARTESI, C ;

RUSSO, M ;

TRIO, R ;

OTERI, G ;

PISANI, F .

THERAPEUTIC DRUG MONITORING, 1992, 14 (06) :509-512

[9] LAMOTRIGINE ANALYSIS IN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

FRASER, AD ;

MACNEIL, W ;

ISNER, AF ;

CAMFIELD, PR .

THERAPEUTIC DRUG MONITORING, 1995, 17 (02) :174-178

[10] LAMOTRIGINE - A REVIEW OF ITS PHARMACOLOGICAL PROPERTIES AND CLINICAL EFFICACY IN EPILEPSY [J].

GOA, KL ;

ROSS, SR ;

CHRISP, P .

DRUGS, 1993, 46 (01) :152-176

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