Oxidation of the two β-carotene molecules in the photosystem II reaction center

被引:46
作者
Telfer, A
Frolov, D
Barber, J
Robert, B
Pascal, A [1 ]
机构
[1] CEA Saclay, Serv Biophys Fonct Membranaires, CEA, DBJC, F-91191 Gif Sur Yvette, France
[2] CEA Saclay, URA 2096, CNRS, F-91191 Gif Sur Yvette, France
[3] Univ London Imperial Coll Sci Technol & Med, Wolfson Labs, Dept Sci Biol, London SW7 2AY, England
关键词
D O I
10.1021/bi026206p
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We present a spectroscopic characterization of the two nonequivalent beta-carotene molecules in the photosystem II reaction center. Their electronic and vibrational properties exhibit significant differences, reflecting a somewhat different configuration for these two cofactors. Both carotenoid molecules are redox-active and can be oxidized by illumination of the reaction centers in the presence of an electron acceptor. The radical cation species show similar differences in their spectroscopic properties. The results are discussed in terms of the structure and unusual function of these carotenoids. In addition, the attribution of resonance Raman spectra of photosystem II preparations excited in the range 800-900 nm is discussed. Although contributions of chlorophyll cations cannot be formally ruled out, our results demonstrate that these spectra mainly arise from the cation radical species of the two carotenoids present in photosystem II reaction centers.
引用
收藏
页码:1008 / 1015
页数:8
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