Method for the noninvasive measurement of intraocular pressure in mice

被引:121
作者
Danias, J
Kontiola, AI
Filippopoulos, T
Mittag, T
机构
[1] CUNY Mt Sinai Sch Med, Dept Ophthalmol, New York, NY 10029 USA
[2] Univ Helsinki, Cent Hosp, Dept Ophthalmol, Helsinki, Finland
关键词
D O I
10.1167/iovs.02-0553
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE. To evaluate the applicability of rebound tonometry for measurement of IOP in the mouse eye. METHODS. An induction-impact (I/I) tonometer, which operates on the rebound principle, was scaled down and adapted to determine IOP of the mouse eye. IOP measurement using this concept is based on contacting the eye with a probe and detecting the motion as the probe collides with the eye and bounces back. The motion parameters of the probe vary according to eye pressure and are used to calculate IOP. A prototype instrument was constructed for measurement of IOP in mouse eyes, and its ability to accurately and reliably measure IOP was tested by comparing the measurements against the manometric (true) IOP determined in cannulated mouse eyes ex vivo. The I/I tonometer was also used to measure IOP in vivo in anesthetized adult C57BL/6 mice. RESULTS. A strong correlation between the true IOP and the I/I measurements (R-2 = 0.95) was found for IOPs in the range of 3.7 to 44.1 mm Hg in cannulated mouse eyes. Repeat determinations in individual eyes showed a low degree of variability in the relationship of the measured IOP with the true IOP. In anesthetized mice, mean IOP +/- SD as determined by rebound tonometry was 9.8 +/- 3.9 mm Hg when the animals were anesthetized with ketamine alone and 7.6 +/- 1.9 mm Hg when a mixture of ketamine, acepromazine, and xylazine was used. Contralateral eyes differed by 0.9 +/- 2.5 and 0.1 +/- 2.7 mm Hg, respectively, for the two anesthetic regimens. CONCLUSIONS. The I/I tonometer can be used for noninvasive, in vivo IOP measurement in mouse eyes. The availability of an easy-to-use, reliable tonometer for IOP measurements in mice will allow more extensive use of the mouse as a model for glaucoma.
引用
收藏
页码:1138 / 1141
页数:4
相关论文
共 28 条
  • [1] Genetic modification of glaucoma associated phenotypes between AKXD-28/Ty and DBA/2J mice
    Anderson, Michael G.
    Smith, Richard S.
    Savinova, Olga V.
    Hawes, Norman L.
    Chang, Bo
    Zabaleta, Adriana
    Wilpan, Robert
    Heckenlively, John R.
    Davisson, Muriel
    John, Simon W. M.
    [J]. BMC GENETICS, 2001, 2 (1)
  • [2] Avila MY, 2001, INVEST OPHTH VIS SCI, V42, P1841
  • [3] A1-, A2A- and A3-subtype adenosine receptors modulate intraocular pressure in the mouse
    Avila, MY
    Stone, RA
    Civan, MM
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 2001, 134 (02) : 241 - 245
  • [4] Cohan BE, 2001, INVEST OPHTH VIS SCI, V42, P340
  • [5] Cohan BE, 2001, INVEST OPHTH VIS SCI, V42, P2560
  • [6] DYNAMIC TONOMETRY
    DEKKING, HM
    COSTER, HD
    [J]. OPHTHALMOLOGICA, 1967, 154 (01) : 59 - &
  • [7] Jia LJ, 2000, INVEST OPHTH VIS SCI, V41, P3415
  • [8] John SWM, 1997, INVEST OPHTH VIS SCI, V38, P249
  • [9] John SWM, 1998, INVEST OPHTH VIS SCI, V39, P951
  • [10] A new electromechanical method for measuring intraocular pressure
    Kontiola, A
    [J]. DOCUMENTA OPHTHALMOLOGICA, 1997, 93 (03) : 265 - 276