A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms

被引：21

作者：

Gmuer, Rudolf ^{[1
]}

Luethi-Schaller, Helga ^{[1
]}

机构：

[1] Univ Zurich, Inst Oral Biol, Sect Oral Microbiol & Gen Immunol, CH-8032 Zurich, Switzerland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 69卷 / 02期

关键词：

16S rRNA; biofilm; fluorescent in situ hybridization; immunofluorescence; dental plaque;

D O I：

10.1016/j.mimet.2006.12.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacteria] populations with distinct 16S rRNA sequences and/or surface markers of interest. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：402 / 405

页数：4

共 20 条

[1] Defining the normal bacterial flora of the oral cavity [J].

Aas, JA ;

Paster, BJ ;

Stokes, LN ;

Olsen, I ;

Dewhirst, FE .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (11) :5721-5732

[2] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[3]

de Lillo A, 2006, ORAL MICROBIOL IMMUN, V21, P61, DOI 10.1111/j.1399-302X.2005.00255.x

[4] PREVALENCE OF BACTEROIDES-FORSYTHUS AND BACTEROIDES-GINGIVALIS IN SUBGINGIVAL PLAQUE OF PROSTHODONTICALLY TREATED PATIENTS ON SHORT RECALL [J].

GMUR, R ;

STRUB, JR ;

GUGGENHEIM, B .

JOURNAL OF PERIODONTAL RESEARCH, 1989, 24 (02) :113-120

[5] Dominant cross-reactive antibodies generated during the response to a variety of oral bacterial species detect phosphorylcholine [J].

Gmür, R ;

Thurnheer, T ;

Guggenheim, B .

JOURNAL OF DENTAL RESEARCH, 1999, 78 (01) :77-85

[6] INTERDENTAL SUPRAGINGIVAL PLAQUE - A NATURAL HABITAT OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, BACTEROIDES-FORSYTHUS, CAMPYLOBACTER-RECTUS, AND PREVOTELLA-NIGRESCENS [J].

GMUR, R ;

GUGGENHEIM, B .

JOURNAL OF DENTAL RESEARCH, 1994, 73 (08) :1421-1428

[7]

Gmür R, 2002, MICROBIOL-SGM, V148, P1379, DOI 10.1099/00221287-148-5-1379

[8]

GMUR R, 1995, VALUE NEW SEROLOGICA

[9] Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients [J].

Hoshino, T ;

Fujiwara, T ;

Kilian, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (12) :6073-6085

[10] The etiology of periodontal disease revisited by population genetic analysis [J].

Kilian, Mogens ;

Frandsen, Ellen V. G. ;

Haubek, Dorte ;

Poulsen, Knud .

PERIODONTOLOGY 2000, 2006, 42 :158-179

← 1 2 →