Increased expression of transient receptor potential vanilloid-1 in airway nerves of chronic cough

被引:295
作者
Groneberg, DA
Niimi, A
Dinh, QT
Cosio, B
Hew, M
Fischer, A
Chung, KF
机构
[1] Univ London Imperial Coll Sci & Technol, Natl Heart & Lung Inst, London SW3 6LY, England
[2] Humboldt Univ, Charite Fac Med, Dept Pediat Pneumol & Immunol, Div Allergy Res, Berlin, Germany
关键词
airway nerves; capsaicin; cough; transient receptor potential vanniloid-1;
D O I
10.1164/rccm.200402-174OC
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Transient receptor potential vanniloid-1 (TRPV-1) mediates the cough response induced by the pepper extract capsaicin and is expressed in sensory nerves that innervate the airway wall. We determined the expression of TRPV-1 in the airways of patients with chronic persistent cough of diverse causes and with an enhanced capsaicin cough response. We obtained airway mucosal biopsies by fiberoptic bronchoscopy in 29 patients with chronic cough and 16 healthy volunteers without a cough. Immunostaining for nerve profiles with anti-protein gene product (PGP)-9.5 antibody showed no increase in nerve profiles in the airway epithelium of patients with chronic cough; however, with an anti-TRPV-1 antibody, there was a fivefold increase of TRPV-1 staining nerve profiles (p < 0.001). There was a significant correlation between capsaicin tussive response and the number of TRPV-1-positive nerves within the patients with cough. Our findings indicate that TRPV-1 receptors may contribute to an enhanced cough reflex and the cough response in chronic persistent cough of diverse causes.
引用
收藏
页码:1276 / 1280
页数:5
相关论文
共 22 条
[1]   The capsaicin receptor: a heat-activated ion channel in the pain pathway [J].
Caterina, MJ ;
Schumacher, MA ;
Tominaga, M ;
Rosen, TA ;
Levine, JD ;
Julius, D .
NATURE, 1997, 389 (6653) :816-824
[2]  
CHOUDRY NB, 1992, EUR RESPIR J, V5, P296
[3]   Cough: potential pharmacological developments [J].
Chung, KF .
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2002, 11 (07) :955-963
[4]   Diagnosis and management of chronic persistent dry cough [J].
Chung, KF ;
Lalloo, UG .
POSTGRADUATE MEDICAL JOURNAL, 1996, 72 (852) :594-598
[5]   Hot channels in airways: pharmacology of the vanilloid receptor [J].
Hwang, SW ;
Oh, U .
CURRENT OPINION IN PHARMACOLOGY, 2002, 2 (03) :235-242
[6]   VR1-immunoreactive primary sensory neurons in the rat trigeminal ganglion [J].
Ichikawa, H ;
Sugimoto, T .
BRAIN RESEARCH, 2001, 890 (01) :184-188
[7]   The diagnosis and treatment of cough [J].
Irwin, RS ;
Madison, JM .
NEW ENGLAND JOURNAL OF MEDICINE, 2000, 343 (23) :1715-1721
[8]   THE SPECTRUM AND FREQUENCY OF CAUSES, KEY COMPONENTS OF THE DIAGNOSTIC EVALUATION, AND OUTCOME OF SPECIFIC THERAPY [J].
IRWIN, RS ;
CURLEY, FJ ;
FRENCH, CL .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1990, 141 (03) :640-647
[9]   Anandamide induces cough in conscious guinea-pigs through VR1 receptors [J].
Jia, YL ;
McLeod, RL ;
Wang, X ;
Parra, LE ;
Egan, RW ;
Hey, JA .
BRITISH JOURNAL OF PHARMACOLOGY, 2002, 137 (06) :831-836
[10]   Acid potentiation of the capsaicin receptor determined by a key extracellular site [J].
Jordt, SE ;
Tominaga, M ;
Julius, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (14) :8134-8139