Accumulation of natural organic matter on the surfaces of living cells: implications for the interaction of toxic solutes with aquatic biota

被引:114
作者
Campbell, PGC
Twiss, MR
Wilkinson, KJ
机构
[1] Univ Quebec, INRS Eau, Ste Foy, PQ G1V 4C7, Canada
[2] Univ Geneva, CABE, CH-1211 Geneva 4, Switzerland
关键词
D O I
10.1139/f97-161
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Reference humic substances (fulvic and humic acid) were used to demonstrate the adsorption of natural dissolved organic matter (DOM) onto the surfaces of phytoplankton (Chlorella pyrenoidosa, Synechococcus leopoliensis) and isolated fish gill cells (Salmo salar-) at environmentally relevant pH values (4-7) and DOM concentrations (0-20 mg . L(-1)) and various ionic strengths (0.02-0.1 M). In the presence of DOM the negative surface charge of cell surfaces increased, as monitored using the electrophoretic mobility of living cells. This effect was much greater at pH 4 than at circumneutral pH, suggesting that the interaction of DOM with cell surfaces involves either a hydrogen-bonding sorption mechanism, between electronegative functional groups present in the DOM and on the cell surface, or the formation of hydrophobic bonds between the cell surface and the hydrophobic domain of the DOM. Sorption of DOM by cell surfaces has been confirmed by direct measurements of DOM loss from solution on contact with phytoplankton cell suspensions and by the observation of the association of humic substances with phytoplankton cell surfaces using transmission electron microscopy. These results demonstrate that DOM interacts with living surfaces and may thereby influence chemical and physical processes at the cell-solution interface.
引用
收藏
页码:2543 / 2554
页数:12
相关论文
共 55 条
[1]   COMPLEXATION BY NATURAL HETEROGENEOUS COMPOUNDS - SITE OCCUPATION DISTRIBUTION-FUNCTIONS, A NORMALIZED DESCRIPTION OF METAL COMPLEXATION [J].
BUFFLE, J ;
ALTMANN, RS ;
FILELLA, M ;
TESSIER, A .
GEOCHIMICA ET COSMOCHIMICA ACTA, 1990, 54 (06) :1535-1553
[2]  
Buffle J., 1988, Complexation Reactions in Aquatic Systems
[3]   Photochemical release of biologically available nitrogen from aquatic dissolved organic matter [J].
Bushaw, KL ;
Zepp, RG ;
Tarr, MA ;
SchulzJander, D ;
Bourbonniere, RA ;
Hodson, RE ;
Miller, WL ;
Bronk, DA ;
Moran, MA .
NATURE, 1996, 381 (6581) :404-407
[4]  
Campbell P.G.C., 1995, Metal Speciation and Bioavailability in Aquatic Systems, P45
[5]   WATER SOLUBILITY ENHANCEMENT OF SOME ORGANIC POLLUTANTS AND PESTICIDES BY DISSOLVED HUMIC AND FULVIC-ACIDS [J].
CHIOU, CT ;
MALCOLM, RL ;
BRINTON, TI ;
KILE, DE .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1986, 20 (05) :502-508
[6]   THE UPTAKE OF MANNITOL AND SORBITOL BY A SPECIES OF CHLORELLA (CHLOROPHYCEAE) [J].
COLMAN, B ;
BRICKELL, PC ;
GEHL, KA .
JOURNAL OF PHYCOLOGY, 1986, 22 (04) :436-440
[7]   IRON REDOX EFFECTS ON PHOTOSENSITIVE PHOSPHORUS RELEASE FROM DISSOLVED HUMIC MATERIALS [J].
COTNER, JB ;
HEATH, RT .
LIMNOLOGY AND OCEANOGRAPHY, 1990, 35 (05) :1175-1181
[8]   ADSORPTION OF NATURAL DISSOLVED ORGANIC-MATTER AT THE OXIDE WATER INTERFACE [J].
DAVIS, JA .
GEOCHIMICA ET COSMOCHIMICA ACTA, 1982, 46 (11) :2381-2393
[9]  
FAIRHURST AJ, 1995, RADIOCHIM ACTA, V69, P103
[10]  
Gamble D. S., 1973, TRACE METALS METAL O, P265