Methods for strand-specific DNA detection with cationic conjugated polymers suitable for incorporation into DNA chips and microarrays

被引:152
作者
Liu, B [1 ]
Bazan, GC
机构
[1] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Dept Chem & Biochem, Santa Barbara, CA 93106 USA
[2] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Dept Mat, Santa Barbara, CA 93106 USA
关键词
peptide nucleic acid probe; polyelectrolytes; fluorescence energy transfer;
D O I
10.1073/pnas.0408561102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A strand-specific DNA sensory method is described based on surface-bound peptide nucleic acids and water-soluble cationic conjugated polymers. The main transduction mechanism operates by taking advantage of the net increase in negative charge at the pepticle nucleic acid surface that occurs upon single-stranded DNA hybridization. Electrostatic forces cause the oppositely charged cationic conjugated polymer to bind selectively to the "complementary" surfaces. This approach circumvents the current need to label the probe or target strands. The polymer used in these assays is poly[9,9'-bis(6"-NNN-trimethylammonium)hexyl)fluoreneco-alt-4,7-(2,1,3-benzothiadiazole) dibromide], which was specifically designed and synthesized to be compatible with excitation sources used in commonly used DNA microarray readers. Furthermore, the utility of poly[9,9'-bis(6"-NN,N-trimethylammonium)hexyl)fluorene-co-alt-4,7-(2,1,3-benzothiadiazole) dibromide] has been demonstrated in homogenous and solid-state assays that involve fluorescence resonance energy transfer to a reporter dye (Cy5) and that can benefit from the light harvesting properties observed in water-soluble conjugated polymers.
引用
收藏
页码:589 / 593
页数:5
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