Choosing an equivalent replacement column for a reversed-phase liquid chromatographic assay procedure

被引：70

作者：

Dolan, JW

Maule, A

Bingley, D

Wrisley, L

Chan, CC

Angod, M

Lunte, C

Krisko, R

Winston, JM

Homeier, BA

McCalley, DV

Snyder, LR

机构：

[1] LC Resources Inc, Orinda, CA 94563 USA

[2] Univ W England, Sch Biomed Sci, Bristol BS16 1QY, Avon, England

[3] Univ Kansas, Dept Chem, Lawrence, KS 66045 USA

[4] Eli Lilly Canada, Toronto, ON, Canada

[5] Wyeth Ayerst Res, Pearl River, NY 10965 USA

[6] 3M Pharmaceut, 3M Ctr, St Paul, MN 55144 USA

[7] BASi NW Lab, McMinnville, OR 97128 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1057卷 / 1-2期

关键词：

column selectivity; selectivity parameters; hydrophobicity; steric resistance; hydrogen-bond acidity; hydrogen-bond basicity; cation-exchange capacity;

D O I：

10.1016/j.chroma.2004.09.064

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The column selectivity parameters (11, S*, A, B and Q described in the preceding paper [L.R. Snyder, A. Maule, A. Heebsch, R. Cuellar, S. Paulson, J. Carrano, L. Wrisley C.C. Chan, N. Pearson, J.W. Dolan, J.J Gilroy, J. Chromatogr. A 1057 (2004) 49-57] can be used to compare columns in terms of selectivity. A detailed procedure for such column comparisons is presented here, and evaluated by its use in finding suitable replacement columns for 12 different routine separations performed in five different pharmaceutical analysis laboratories. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：59 / 74

页数：16

共 13 条

[1] Variability of column selectivity for reversed-phase high-performance liquid chromatography - Compensation by adjustment of separation conditions [J].