Glutathione transferase M2-2 catalyzes conjugation of dopamine and dopa o-quinones

被引：86

作者：

Dagnino-Subiabre, A

Cassels, BK

Baez, S

Johansson, AS

Mannervik, B

Segura-Aguilar, J

机构：

[1] Univ Chile, Fac Med, ICBM, Programme Mol & Clin Pharmacol, Santiago 7, Chile

[2] Univ Chile, Fac Sci, Dept Chem, Santiago, Chile

[3] Uppsala Univ, Biomed Ctr, Dept Biochem, SE-75123 Uppsala, Sweden

[4] Stockholm Univ, Wallenberg Lab, Biochem Toxicol Unit, SE-10691 Stockholm, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 274卷 / 01期

关键词：

dopamine; dopa; glutathione transferase; o-quinones; aminochrome; dopachrome; 5-S-cysteinyl-dopa; 5-S-cysteinyldopamine; neuromelanin;

D O I：

10.1006/bbrc.2000.3087

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human glutathione transferase M2-2 prevents the formation of neurotoxic aminochrome and dopachrome by catalyzing the conjugation of dopamine and dopa o-quinone with glutathione. NMR analysis of dopamine and dopa o-quinone-glutathione conjugates revealed that the addition of glutathione was at C-5 to form 5-S-glutathionyl-dopamine and 5-S-glutathionyldopa, respectively. Both conjugates were found to be resistant to oxidation by biological oxidizing agents such as O-2, H2O2, and O-2(.-), and the glutathione transferase-catalyzed reaction can therefore serve a neuroprotective antioxidant function. (C) 2000 Academic Press.

引用

页码：32 / 36

页数：5

共 23 条

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