In vivo modulation of a DnaJ homolog, CbpA, by CbpM

被引：16

作者：

Chenoweth, Matthew R.

Trun, Nancy

Wickner, Sue

机构：

[1] NCI, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

[2] Duquesne Univ, Dept Biol Sci, Pittsburgh, PA 15282 USA

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 09期

关键词：

D O I：

10.1128/JB.01757-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CbpA, an Escherichia coli DnaJ homolog, can function as a cochaperone for the DnaK/Hsp70 chaperone system, and its in vitro activity can be modulated by CbpM. We discovered that CbpM specifically inhibits the in vivo activity of CbpA, preventing it from functioning in cell growth and division. Furthermore, we have shown that CbpM interacts with CbpA in vivo during stationary phase, suggesting that the inhibition of activity is a result of the interaction. These results reveal that the activity of the E. coli DnaK system can be regulated in vivo by a specific inhibitor.

引用

收藏

页码：3635 / 3638

页数：4

相关论文

共 18 条

[1]

ALI AT, 1999, J BACTERIOL, V181, P6361

[2] Functional analysis of CbpA, a DnaJ homolog and nucleoid-associated DNA-binding protein [J].

Bird, Jeremy G. ;

Sharma, Suveena ;

Roshwalb, Sara C. ;

Hoskins, Joel R. ;

Wickner, Sue .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (45) :34349-34356

[3] The Hsp70 and Hsp60 chaperone machines [J].

Bukau, B ;

Horwich, AL .

CELL, 1998, 92 (03) :351-366

[4] CbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM [J].

Chae, C ;

Sharma, S ;

Hoskins, JR ;

Wickner, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (32) :33147-33153

[5] DafA cycles between the DnaK chaperone system and translational machinery [J].

Dumitru, GL ;

Groemping, Y ;

Klostermeier, D ;

Restle, T ;

Deuerling, E ;

Reinstein, J .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 339 (05) :1179-1189

[6] A cis-acting sequence involved in chromosome segregation in Escherichia coli [J].

Fekete, RA ;

Chattoraj, DK .

MOLECULAR MICROBIOLOGY, 2005, 55 (01) :175-183

[7] The djlA gene acts synergistically with dnaJ in promoting Escherichia coli growth [J].

Genevaux, P ;

Schwager, F ;

Georgopoulos, C ;

Kelley, WL .

JOURNAL OF BACTERIOLOGY, 2001, 183 (19) :5747-5750

[8] All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins [J].

Gur, E ;

Katz, C ;

Ron, EZ .

FEBS LETTERS, 2005, 579 (09) :1935-1939

[9] TIGHT REGULATION, MODULATION, AND HIGH-LEVEL EXPRESSION BY VECTORS CONTAINING THE ARABINOSE P-BAD PROMOTER [J].

GUZMAN, LM ;

BELIN, D ;

CARSON, MJ ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1995, 177 (14) :4121-4130

[10] Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter [J].

Khlebnikov, A ;

Datsenko, KA ;

Skaug, T ;

Wanner, BL ;

Keasling, JD .

MICROBIOLOGY-SGM, 2001, 147 :3241-3247