A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates

被引:159
作者
Medintz, Igor L. [1 ]
Berti, Lorenzo
Pons, Thomas
Grimes, Amy F.
English, Douglas S.
Alessandrini, Andrea
Facci, Paolo
Mattoussi, Hedi
机构
[1] USN, Res Lab, Div Opt Sci, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA
[2] INFM, CNR, Natl Res Ctr NanoStruct & BioSyst Surfaces, S3, I-41100 Modena, Italy
[3] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
关键词
D O I
10.1021/nl070782v
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Self-assembly of proteins, peptides, DNA, and other biomolecules to semiconductor quantum dots (QD) is an attractive bioconjugation route that can circumvent many of the problems associated with covalent chemistry and subsequent purification. Polyhistidine sequences have been shown to facilitate self-assembly of proteins and peptides to ZnS-overcoated CdSe QDs via complexation to unoccupied coordination metal sites on the nanocrystal surface. We describe the synthesis and characterization of a thiol-reactive hexahistidine peptidic linker that can be chemically attached to thiolated-DNA oligomers and mediate their self-assembly to CdSe-ZnS core-shell QDs. The self-assembly of hexahistidine-appended DNA to QDs is probed with gel electrophoresis and fluorescence resonance energy transfer techniques, and the results confirm high-affinity conjugate formation with control over the average molar ratio of DNA assembled per QD. To demonstrate the potential of this reactive peptide linker strategy, a prototype QD-DNA-dye molecular beacon is self-assembled and tested against both specific and nonspecific target DNAs. This conjugation route is potentially versatile, as altering the reactivity of the peptide linker may allow targeting of different functional groups such as amines and facilitate self-assembly of other nanoparticle-biomolecule structures.
引用
收藏
页码:1741 / 1748
页数:8
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