Near-Infrared Light Emitting Luciferase via Biomineralization

被引：91

作者：

Ma, Nan ^{[1
]}

Marshall, Ann F. ^{[4
]}

Rao, Jianghong ^{[1
,2
,3
]}

机构：

[1] Stanford Univ, MIPS, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Radiol, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[4] Stanford Univ, Geballe Lab Adv Mat, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 20期

关键词：

NANOCRYSTALS; BIOLUMINESCENCE;

D O I：

10.1021/ja101378g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new strategy based on biomineralization is presented to rationally tune the emission wavelength of luciferase. In this study luciferase (Luc8) was used as a template to direct the synthesis of near-infrared (NIR) light emitting PbS quantum dots (QDs) at ambient conditions to form a Luc8-PbS nanocomplex. The as-synthesized PbS QDs exhibited photoluminescence in the range of 800-1050 nm, and the Luc8 enzyme remained active within the Luc8-PbS complex. Upon the addition of the substrate coelenterazine, the energy produced by Luc8 was nonradiatively transferred to PbS QDs via bioluminescence resonance energy transfer (BRET) and enabled the complex to emit NIR light. This is the first study to form dual functional bioinorganic hybrid nanostructures via active enzyme-templated synthesis of inorganic nanomaterials.

引用

页码：6884 / +

页数：3

共 19 条

[1]

[Anonymous], 2001, Biomineralization: principles and concepts in bioinorganic materials chemistry

[2] Polysaccharides and Proteoglycans in Calcium Carbonate-based Biomineralization [J].