Endothelial cell-mediated regulation of aldosterone release from human adrenocortical cells

被引:18
作者
Ansurudeen, Ishrath
Kopprasch, Steffi
Ehrhart-Bornstein, Monika
Bornstein, Stefan R.
Willenberg, Holger S.
机构
[1] Tech Univ Dresden, Carl Gustav Carus Med Sch, Med Clin 3, Dept Med 3, D-01307 Dresden, Germany
[2] Univ Dusseldorf, Dept Endocrinol Diabet & Rheumatol, D-40225 Dusseldorf, Germany
关键词
HUVEC; endothelial cells; aldosterone; Cyp11B2 (aldosterone synthase); CREB; CRE; StAR; SF-1;
D O I
10.1016/j.mce.2006.12.011
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Endothelial cells play an important role in the development and functioning of endocrine tissue and endothelial cell-derived factors have been shown to regulate mineralocorticoid release in bovine adrenal cells. In the present study, we analysed the role of human endothelial cells in the synthesis and release of aldosterone from adrenocortical cells (NCI-H295R). Endothelial cell-induced aldosterone release was rapid and lasted as a long-term effect over a period of 48 h. This stimulant effect was influenced by the duration of endothelial cell conditioning and decreased linearly with increasing dilutions of the conditioned medium. At the molecular level, an increase in the mRNA transcripts of aldosterone synthase and StAR could be observed. Cellular interaction with endothelial cell-factors enhanced the activation of CRE, and the promoter activity of both StAR and SF-1 reporter genes. In conclusion, human endothelial cells are important intra-adrenal regulators of human aldosterone synthesis and release. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:150 / 156
页数:7
相关论文
共 35 条
  • [1] HYPERRENINEMIC HYPOALDOSTERONISM AFTER CHRONIC STRESS IN THE RAT
    AGUILERA, G
    KISS, A
    SUNARAKBASAK, B
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (03) : 1512 - 1519
  • [2] Vascular-adrenal niche - Endothelial cell-mediated sensitization of human adrenocortical cells to angiotensin II
    Ansurudeen, I.
    Kopprasch, S.
    Ehrhart-Bornstein, M.
    Willenberg, H. S.
    Krug, A. W.
    Funk, R. H. W.
    Bornstein, S. R.
    [J]. HORMONE AND METABOLIC RESEARCH, 2006, 38 (07) : 476 - 480
  • [3] Bassett JR, 1997, MICROSC RES TECHNIQ, V36, P546, DOI 10.1002/(SICI)1097-0029(19970315)36:6<546::AID-JEMT11>3.0.CO
  • [4] 2-O
  • [5] The regulation of aldosterone synthase expression
    Bassett, MH
    White, PC
    Rainey, WE
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2004, 217 (1-2) : 67 - 74
  • [6] HUMAN NCI-H295 ADRENOCORTICAL CARCINOMA-CELLS - A MODEL FOR ANGIOTENSIN-II-RESPONSIVE ALDOSTERONE SECRETION
    BIRD, IM
    HANLEY, NA
    WORD, RA
    MATHIS, JM
    MCCARTHY, JL
    MASON, JI
    RAINEY, WE
    [J]. ENDOCRINOLOGY, 1993, 133 (04) : 1555 - 1561
  • [7] ABSENCE OF PROSTACYCLIN INVOLVEMENT IN ANGIOTENSIN-INDUCED ALDOSTERONE SECRETION IN RAT ADRENAL-CELLS
    CAMPBELL, WB
    GOMEZSANCHEZ, CE
    [J]. ENDOCRINOLOGY, 1985, 117 (01) : 279 - 286
  • [8] Characterization of the promoter region of the mouse gene encoding the steroidogenic acute regulatory protein
    Caron, KM
    Ikeda, Y
    Soo, SC
    Stocco, DM
    Parker, KL
    Clark, BJ
    [J]. MOLECULAR ENDOCRINOLOGY, 1997, 11 (02) : 138 - 147
  • [9] The steroidogenic acute regulatory protein is induced by angiotensin II and K+ in H295R adrenocortical cells
    Clark, BJ
    Pezzi, V
    Stocco, DM
    Rainey, WE
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1995, 115 (02) : 215 - 219
  • [10] Angiotensin II and potassium regulate human CYP11B2 transcription through common cis-elements
    Clyne, CD
    Zhang, Y
    Slutsker, L
    Mathis, JM
    White, PC
    Rainey, WE
    [J]. MOLECULAR ENDOCRINOLOGY, 1997, 11 (05) : 638 - 649