Multimode detection (LA-ICP-MS, MALDI-MS and nanoHPLC-ESI-MS2) in 1D and 2D gel electrophoresis for selenium-containing proteins

被引：37

作者：

Ballihaut, Guillaume

Pecheyran, Christophe

Mounicou, Sandra

Preud'homme, Hugues

Grimaud, Regis

Lobinski, Ryszard

机构：

[1] Laboratoire de Chimie Analytique Bio-inorganique et Environnement, UMR 5034, Hélioparc, 2, av. Pr. Angot

[2] Laboratoire d'Ecologie Moléculaire (Microbiologie), av. de l'Université

[3] Department of Analytical Chemistry, Warsaw University of Technology, ul. Noakowskiego 3

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2007年 / 26卷 / 03期

关键词：

gel electrophoresis; inductively-coupled plasma; laser ablation; mass spectrometry; multimode detection; nano high-performance liquid chromatography; protein; selenium; tandem mass spectrometry;

D O I：

10.1016/j.trac.2007.01.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Speciation of heteroelement-containing proteins essential for human health is one of the most rapidly developing areas in bio-inorganic analytical chemistry (metallomics). We discuss recent advances in mass spectrometry (MS) techniques for detection and identification of selenium (Se)-containing proteins separated by gel electrophoresis (GE). These advances include location of Se-containing bands in 1D GE or spots in 2D GE by laser-ablation inductively coupled plasma MS (LA-ICP-MS) followed by protein identification by matrix-assisted laser-desorption/ionization MS (MALDI-MS) and nano-high-performance liquid chromatography-electrospray-tandem MS (nanoHPLC-ESI-MS 2 ). We highlight the differences with the classical proteomics approach resulting from the presence of the Se atom. © 2007 Elsevier Ltd. All rights reserved.

引用

页码：183 / 190

页数：8

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