Fate of fructose supplied to leaf sheaths after defoliation of Lolium perenne L.:: assessment by 13C-fructose labelling

被引:25
作者
Amiard, V [1 ]
Morvan-Bertrand, A [1 ]
Billard, JP [1 ]
Huault, C [1 ]
Prud'homme, MP [1 ]
机构
[1] Univ Caen, UMR, INRA, UCBN Physiol & Biochim Vegetales,IRBA, F-14032 Caen, France
关键词
fructans; glucose; leaf growth zone; loliose; regrowth; reserves; roots; sink strength; sucrose;
D O I
10.1093/jxb/erg125
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The role of fructans from leaf sheaths for the refoliation of Lolium perenne after severe defoliation was assessed by following the fate of C-13-fructose supplied to leaf sheaths at the time of defoliation. At the end of the 4 h labelling period on defoliated plants, 77% of the C-13 incorporated was still located in leaf sheaths. Only 4% and 0.9% were, respectively, allocated to stem and roots, while 18% was imported by the growing leaves where C-13 was allocated first to the proximal part of the leaf growth zone (0-10 mm). In all tissues, the most highly C-13-labelled carbohydrates was not fructose but sucrose. In leaf sheaths, C-13-loliose was produced. In the leaf growth zone (0-20 mm), fructans were simultanously synthesized from C-13 entering the leaves and degraded. The export of C-13 from leaf sheaths continued during the first day of regrowth but stopped afterwards. There was no net loss of C from C-13-fructose over the first 2 d of regrowth. The role of fructans and lollose is discussed as well as the physiological mechanisms contributing to defoliation tolerance in L. perenne.
引用
收藏
页码:1231 / 1243
页数:13
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