An improved method for recovering rabies virus from cloned cDNA

被引：109

作者：

Inoue, K

Shoji, Y

Kurane, I

Iijima, T

Sakai, T

Morimoto, K

机构：

[1] Natl Inst Infect Dis, Dept Virol 1, Shinjuku Ku, Tokyo 1628640, Japan

[2] Tohoku Univ, Grad Sch Life Sci, Dept Dev Biol & Neurosci, Div Syst Neurosci,Aoba Ku, Sendai, Miyagi 9808577, Japan

[3] Nihon Univ, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, Kanagawa 2528510, Japan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2003年 / 107卷 / 02期

关键词：

rabies virus; HEP-Flury strain; reverse genetics; cytomegalovirus promotor;

D O I：

10.1016/S0166-0934(02)00249-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new system for recovery of rabies virus from cDNA plasmid, the transcription of which was driven by cellular RNA polymerase II, was developed. The plasmid contains full-length viral cDNA flanked by hammerhead ribozyme and hepatitis delta ribozyme sequences, arranged downstream of the cytomegalovirus (CMV) promotor. Transfection with the full-length cDNA plasmid together with helper plasmids encoding viral N, P, and L proteins without supply of T7 RNA polymerase produced a recombinant rabies virus in several cell lines. The efficiency of recovery between the conventional T7 promotor system and the new CMV promotor system was compared using these plasmid constructs. The newly established system is applicable to various cell lines and allows rapid and efficient generation of recombinant rabies virus. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：229 / 236

页数：8

相关论文

共 31 条

[1] Identification of a phosphatase-sensitive epitope of rabies virus nucleoprotein which is recognized by a monoclonal antibody 5-2-26 [J].

Anzai, J ;

Takamatsu, F ;

Takeuchi, K ;

Kohno, T ;

Morimoto, K ;

Goto, H ;

Minamoto, N ;

Kawai, A .

MICROBIOLOGY AND IMMUNOLOGY, 1997, 41 (03) :229-240

[2] Self-cleaving ribozymes of hepatitis delta virus RNA [J].

Been, MD ;

Wickham, GS .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 247 (03) :741-753

[3] Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter [J].

Buchholz, UJ ;

Finke, S ;

Conzelmann, KK .

JOURNAL OF VIROLOGY, 1999, 73 (01) :251-259

[4] Infection characteristics of rabies virus variants with deletion or insertion in the pseudogene sequence [J].

Ceccaldi, PE ;

Fayet, J ;

Conzelmann, KK ;

Tsiang, H .

JOURNAL OF NEUROVIROLOGY, 1998, 4 (01) :115-119

[5] PRODUCTION OF INFECTIOUS HUMAN RESPIRATORY SYNCYTIAL VIRUS FROM CLONED CDNA CONFIRMS AN ESSENTIAL ROLE FOR THE TRANSCRIPTION ELONGATION-FACTOR FROM THE 5'-PROXIMAL OPEN READING FRAME OF THE M2 MESSENGER-RNA IN GENE-EXPRESSION AND PROVIDES A CAPABILITY FOR VACCINE DEVELOPMENT [J].

COLLINS, PL ;

HILL, MG ;

CAMARGO, E ;

GROSFELD, H ;

CHANOCK, RM ;

MURPHY, BR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (25) :11563-11567

[6] CHARACTERIZATION OF AN ANTIGENIC DETERMINANT OF THE GLYCOPROTEIN THAT CORRELATES WITH PATHOGENICITY OF RABIES VIRUS [J].

DIETZSCHOLD, B ;

WUNNER, WH ;

WIKTOR, TJ ;

LOPES, AD ;

LAFON, M ;

SMITH, CL ;

KOPROWSKI, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :70-74

[7] ANALYSIS OF MUTATION IN HUMAN-CELLS BY USING AN EPSTEIN-BARR-VIRUS SHUTTLE SYSTEM [J].

DUBRIDGE, RB ;

TANG, P ;

HSIA, HC ;

LEONG, PM ;

MILLER, JH ;

CALOS, MP .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :379-387

[8]

Flamand A, 1993, Trends Microbiol, V1, P317, DOI 10.1016/0966-842X(93)90010-O

[9] Rescue of influenza A virus from recombinant DNA [J].

Fodor, E ;

Devenish, L ;

Engelhardt, OG ;

Palese, P ;

Brownlee, GG ;

García-Sastre, A .

JOURNAL OF VIROLOGY, 1999, 73 (11) :9679-9682

[10] EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].

FUERST, TR ;

NILES, EG ;

STUDIER, FW ;

MOSS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126

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