MTO:: the second member of a Drosophila dual copper-thionein system

被引：30

作者：

Domenech, J

Palacios, O

Villarreal, L

González-Duarte, P

Capdevila, M

Atrian, S

机构：

[1] Univ Barcelona, Fac Biol, Dept Genet, E-08028 Barcelona, Spain

[2] Univ Autonoma Barcelona, Fac Ciencias, Dept Quim, E-08193 Barcelona, Spain

来源：

FEBS LETTERS | 2003年 / 533卷 / 1-3期

关键词：

Drosophila metallothionein; Cu(I) binding; Cd(II) binding; Zn(II) binding; molecular evolution; recombinant synthesis;

D O I：

10.1016/S0014-5793(02)03754-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：72 / 78

页数：7

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