Inhibition of macrophage nitric oxide production by gangliosides derived from a spontaneous T cell lymphoma: the involved mechanisms

被引：33

作者：

Bharti, AC

Singh, SM

机构：

[1] Univ Texas, MD Anderson Canc Ctr, Dept Bioimmunotherapy, Cytokine Res Lab, Houston, TX 77030 USA

[2] Banaras Hindu Univ, Sch Biotechnol, Varanasi 221005, Uttar Pradesh, India

来源：

NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2003年 / 8卷 / 01期

关键词：

Dalton's lymphoma; gangliosides; macrophage activation; inducible nitric oxide synthase; immunosuppression;

D O I：

10.1016/S1089-8603(02)00145-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Gangliosides (DLG) derived from a spontaneous T cell lymphoma (Dalton's lymphoma) have been shown to impair the ability of lipopolysaccharide-activated macrophages to produce nitric oxide (NO). However, the mechanism and nature of this effect is not known. In this investigation, we sought to (1) determine whether the inhibitory action of DLG on macrophages is through the modulation of inducible nitric oxide synthase (iNOS) expression and (2) identify the possible mechanisms and signal transduction events underlying the inhibitory action of DLG. Immunoblot analysis of DLG-treated macrophages showed a decrease in iNOS expression. DLG also inhibited the production of monokines interleukin-1 and tumor necrosis factor by macrophages. However, the DLG-induced inhibition was reversible in nature. Studies showed that DLG-induced inhibition of macrophage activation could be blocked by sodium orthovanadate, indicating a role of phosphatase activity in ganglioside-induced inhibition. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：75 / 82

页数：8

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